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Title: | Paediatric rotavirus diarrhoea in Sri Lanka: a preliminary report |
Authors: | Chandrasena, T.G.A.N. Rajindrajith, S. Ahmed, K. Pathmeswaran, A. Abeyewickreme, W. Nakagomi, O. |
Keywords: | Rotavirus Infections Deal Barratt Dillon syndrome |
Issue Date: | 2007 |
Publisher: | Sri Lanka College of Paediatricians |
Citation: | Proceedings of 12th Asia Pacific Congress of Paediatrics and 2nd Asia Pacific Congress of Paediatric Nursing. 2007; 1(1): 44 |
Abstract: | BACKGROUND: Group A rotavirus is the leading cause of acute gastroenteritis in children. Serotypes Gl, G2, G3 and G4 are mainly responsible for human infections. Strain characterization and serotype distribution of rotavirus in a country is an importaa determinant of future vaccine strategy. Information in this regard is scarce in Sri Lanka. OBJECTIVES: To determine the prevalence, severity and molecular epidemiology of rotavirus diarrhoea among children hospitalized with diarrhoea in Sri Lanka. DESIGN, SETTING AND METHOD: A prospective hospital-based study was conducted in the paediatric units of the North Colombo Teaching Hospital from April 2005-February 2006. Stool samples of children admitted with diarrhoea were analyzed for Group A rotavirus antigen by enzyme linked immunosorbent assay (EL1SA) (Rotaclone). Samples positive for rotavirus were characterized electropherotyping (PAGE) and serotyping (reverse transcription-poiymerase chain reaction (RT-PCR)) respectively. Severity of diarrhoea was assessed by the Vesikari severity score. RESULTS: A total of 341 children [204 males mean age 25.7 months (range 1-144)] were studied. Sixty seven (19.6%) had rotavirus diarrhoea. RT-PCR and PAGE were done on 58 rotavirus positive samples. Thirty one were PAGE positive with 6 different electropherotypes. RT-PCR revealed the presence of serotypes Gl, G2, G3, G4 and G9 in 7 (12.1%), 16 (27.6%),2 (3.4%), 2 (3.4%), and 11 (19.0%) samples respectively. Twenty (34.5%) were untypable. Severity score, assessed in 326 patients, revealed a mean score of 13.3 and 11.4 in rotavirus positive and negative patients respectively (p=0.05). Presence frequency and duration of vomiting and duration of diarrhoea were significantly higher in rotavirus diarrhoea (p<0.05). CONCLUSIONS: Rotavirus is an important agent of severe paediatric diarrhoea in Sri Lanka. Molecular analysis indicates genetic diversity among group A rotavirus in Sri Lanka. This study reports for the first time of G9 type rotavirus infection in Sri Lanka. |
Description: | Oral Presentation Abstracts (OP09)10th Annual Congress of Sri Lanka College of Paediatricians, 12th to 15h March, 2007 Colombo, Sri Lanka |
URI: | http://repository.kln.ac.lk/handle/123456789/11511 |
ISSN: | 1391-2992 |
Appears in Collections: | Conference Papers |
Files in This Item:
File | Description | Size | Format | |
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PAEDIATRIC ROTAVIRUS DIARRHOEA IN SRI LANKA.pdf | 96.82 kB | Adobe PDF | View/Open |
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