Cloning and characterization of a repetitive DNA sequence specific for Wuchereria bancrofti
dc.contributor.author | Siridewa, K. | en_US |
dc.contributor.author | Karunanayake, E.H. | en_US |
dc.contributor.author | Chandrasekharan, N.V. | en_US |
dc.contributor.author | Abeyewickreme, W. | en_US |
dc.contributor.author | Franzen, L. | en_US |
dc.contributor.author | Aslund, L. | en_US |
dc.contributor.author | Pettersson, U. | en_US |
dc.creator.corporateauthor | American Society of Tropical Medicine and Hygiene | en_US |
dc.date.accessioned | 2014-10-29T09:11:41Z | |
dc.date.available | 2014-10-29T09:11:41Z | |
dc.date.issued | 1994 | en_US |
dc.description | Indexed in MEDLINE | |
dc.description.abstract | A genomic library constructed in a bacteriophage lambda replacement vector (EMBL3) with Wuchereria bancrofti DNA partially digested with Sau 3A I was screened with 32P-labeled W. bancrofti total DNA, and a strongly reactive recombinant, EMBL3Wb34, was isolated. This clone contained an approximately 16-kb insert that showed some cross-hybridization with Brugia malayi and B. pahangi DNA. However, a 969-bp subclone from EMBL3Wb34, designated pWb12, hybridized only with W. bancrofti DNA and was able to detect as little as 300 pg. Furthermore, pWb12 could detect DNA from a single infective larva or one microfilaria. It has a moderate copy number (450-700) and appears to be interspersed within the parasite genome. The nucleotide sequence contains 66% A+T and 34% G+C and shows no notable internal repeats. | en_US |
dc.identifier.citation | The American Journal of Tropical Medicine and Hygiene. 1994; 51(4): pp.495-500 | en_US |
dc.identifier.department | Parasitology | en_US |
dc.identifier.issn | 0002-9637 (Print) | en_US |
dc.identifier.issn | 1476-1645 (Electronic) | en_US |
dc.identifier.uri | http://repository.kln.ac.lk/handle/123456789/1228 | |
dc.publisher | American Society of Tropical Medicine and Hygiene | en_US |
dc.subject | DNA, Helminth-chemistry | en_US |
dc.subject | Repetitive Sequences, Nucleic Acid | en_US |
dc.subject | Wuchereria bancrofti-genetics | en_US |
dc.subject | Wuchereria bancrofti | |
dc.title | Cloning and characterization of a repetitive DNA sequence specific for Wuchereria bancrofti | en_US |
dc.type | Article | en_US |
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