Potential of bio fabricated Annona glabra - silver nanoparticles to control Aedes aegypti and Aedes albopictus
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Date
2020
Journal Title
Journal ISSN
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, Proceedings of the 1st International Conference on Frontiers in Chemical Technology, Institute of Chemistry Ceylon
Abstract
Bio synthesized silver nanoparticles using Annona glabra leaf extract (An-AgNPs) as the reducing and stabrlizing agent were assessed as a novel larvicide against dengue vector mosquito larvae. The fresh leaves of A. glabra were crushed well and heated with distilled water under 70 0C for I hour. The supernatant was mixed (10% and 20% v/v of total volume) with 5 mM AgNO, to synthesize AnAgNPs. Formation ofAn-AgNPs were confirmed bythe color change and by appearance ofSPR peak in the Uv-vis spectrum and characterized by Dynamic Light Scattering (DLS), Scanning Electron Microscopy (SEM) and Fourier Transform Infrared spectroscopy (FTIR). lhe larvicidal activity of An-AgNPs was assessed against the third instar larvae of Ae. aegypti ^nd, Ae. albopictus, under the laboratory conditions (2 -10 mg/L). The change ofsolution color from yellow to dark brown/black and the SPR band at 435 nm confirmed the formation of An-AgNPs. According to SEM; An-AgNPs were spherical in morphology. FTIR analysis detected that An-AgNPs are capped and stabilized with biomolecules of A. glabraleaf ltZl9 (C=O stretching), 1366 (CH2 bending) and 3500-3200 (N-H and O-H stretching) and l2l7 cm-' (C-O stretching)i. The LCro of An-AgNPs (10%) and An-AgNPs (20%)againstthethirdinstarslarvaeofAe.aeglptiafter24hourswere5.2gand2.43mglland of Ae. albopictus were 3.02 and 2.51 mg/L respectively. The LC* of An-AgNPs (I0%) and An-AgNPs (20%)againstthethirdinstarslarvaeof Ae.aegyptiafter48hourswerel.5l andl.lT mg/Landof Ae. albopictus were 1.14 and 2.i0 mg/L respectively. The LC,n ofleafcrude plant extract against the third instars larvae of Ae. aegTpti after 24 and 48 hours were 5.9455 and 3.5485 mg/L and for the third instars iaruae of Ae. albopictus were 5.0040 arrd2.7346 mg/L respectivdy. This study shows that AnAgNPs is efficient in controlling mosquito larvae.
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Keywords
A. aegypti, A. albopictus, A. glabra, Larvicidal activity, Ag nanoparticles
Citation
Aberathna, A.A.A.U., Amarasinghe, L.D., Sithara, W.S., Wickramarachchi, S. and De Silva, C.R. (2020). Potential of bio fabricated Annona glabra - silver nanoparticles to control Aedes aegypti and Aedes albopictus, Proceedings of the 1st International Conference on Frontiers in Chemical Technology, Institute of Chemistry Ceylon, P.119