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Antibacterial Polyketide from Lasiodiplodia theobromae and Pyrenula bahiana on Mangrove Ecosystems in Puttalam Lagoon, Sri Lanka

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dc.contributor.author Paranagama, P.A.
dc.contributor.author Santhirasegaram, S.
dc.contributor.author Wickramarachchi, S.R.
dc.contributor.author Attanayake, R.N.
dc.contributor.author Weerakoon, G.
dc.contributor.author Maduranga, K.
dc.date.accessioned 2022-10-26T07:45:01Z
dc.date.available 2022-10-26T07:45:01Z
dc.date.issued 2022
dc.identifier.citation Paranagama, P., Santhirasegaram, S., Wickramarachchi, S., Attanayake, R., Weerakoon, G., & Maduranga, K. (2022). Antibacterial Polyketide from Lasiodiplodia theobromae and Pyrenula bahiana on Mangrove Ecosystems in Puttalam Lagoon, Sri Lanka. Asian Journal of Chemistry, 34(11), 2961–2964. https://doi.org/10.14233/ajchem.2022.23789 en_US
dc.identifier.uri http://repository.kln.ac.lk/handle/123456789/25336
dc.description.abstract Lasiodiplodia theobromae is one of the frequently isolated fast growing endolichenic fungus. This fungus was isolated from the lichen host, Pyrenula bahiana collected from the mangrove ecosystems in Puttlam lagoon and its identification was confirmed based on rDNA-ITS sequence homology. Secondary metabolites of L. theobromae were extracted into ethyl acetate and subjected to antibacterial assay against Escherichia coli (ATCC25922), Staphylococcus aureus (ATCC25923) and Bacillus subtilis (ATCC6051). Crude extract at a concentration of 6.8 μg/mL showed good antibacterial activity against the bacterial strain S. aureus compared with the activity of the standard azithromycin at a concentration of 5.0 μg/mL. Active crude extract was partitioned to obtain methanol, hexane and chloroform fractions. Chloroform fraction showed the highest activity to S. aureus out of three fractions. This fraction was subjected to bioassay-guided separation on silica gel column chromatography to isolate bioactive pure compounds. The bioactive pure compound was identified as (3R)-de-O-methyllasiodiplodin using LC-MS, 1D and 2D NMR spectroscopy. en_US
dc.publisher Asian Journal of Chemistry en_US
dc.subject Endolichenic fungi, Mangrove systems, Antibacterial activity, Staphylococcus aureus, (3R)-de-O-methyllasiodiplodin. en_US
dc.title Antibacterial Polyketide from Lasiodiplodia theobromae and Pyrenula bahiana on Mangrove Ecosystems in Puttalam Lagoon, Sri Lanka en_US


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