Digital Repository

A Novel Cytotoxic Compound From the Endolichenic Fungus, Xylaria psidii Inhabiting the Lichen, Amandinea medusulina

Show simple item record

dc.contributor.author Santhirasegaram, S.
dc.contributor.author Wickramarachchi, S. R.
dc.contributor.author Attanayake, R. N.
dc.contributor.author Weerakoon, G.
dc.contributor.author Samarakoon, S.
dc.contributor.author Wijeratne, K.
dc.contributor.author Paranagama, P. A.
dc.date.accessioned 2021-10-31T20:04:44Z
dc.date.available 2021-10-31T20:04:44Z
dc.date.issued 2020
dc.identifier.citation Santhirasegaram, S., Wickramarachchi, S. R., Attanayake, R. N., Weerakoon, G., Samarakoon, S., Wijeratne, K., & Paranagama, P. A. (2020). A Novel Cytotoxic Compound From the Endolichenic Fungus, Xylaria psidii Inhabiting the Lichen, Amandinea medusulina. Natural Product Communications. https://doi.org/10.1177/1934578X20933017 en_US
dc.identifier.uri http://repository.kln.ac.lk/handle/123456789/23655
dc.description.abstract The lichen host, Amandinea medusulina, collected from mangrove habitats in Sri Lanka, and its associated endolichenic fungi were isolated and identified by rDNA-ITS sequence analysis and morphological features. One of the fungal strains frequently isolated from the lichen thalli was identified as Xylaria psidii. This study aimed at the isolation and identification of the cytotoxic compounds present in this fungus. Secondary metabolites of X. psidii were first extracted into ethyl acetate and subsequently subjected to bioassay-guided fractionation to isolate the bioactive compounds. Sulforhodamine B assay against a lung cancer (NCI-H292) cell line was used to determine the differential cytotoxic activity. Bioassay-guided fractionation led to the isolation of an active compound, SS/02/29/08, showing moderate cytotoxicity (IC50 = 27.2 μg/mL). Its structure was elucidated by IR, 1D- and 2D-NMR, and 13C-NMR spectrophotometry and MS, in combination with HRMS, 13C NMR, HSQC, HMBC, and DQF-COSY. The structure of SS/02/29/08 was determined as (Z)-3-{(3-acetyl- 2-hydroxyphenyl) diazenyl}-2,4-dihydroxybenzaldehyde and identified as a new compound. This novel compound has promising differential cytotoxic activity against human lung cancer cell line (NCI-H292). en_US
dc.publisher Natural Product Communications en_US
dc.subject endolichenic fungi, Xylaria psidii, Amandinea medusulina, anticancer activity, lung cancer, bioactivity en_US
dc.title A Novel Cytotoxic Compound From the Endolichenic Fungus, Xylaria psidii Inhabiting the Lichen, Amandinea medusulina en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search Digital Repository


Browse

My Account