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Fungal pretreatment to enhance the yield of phytochemicals and evaluation of α-amylase and α- glucosidase inhibition using Cinnamomum zeylanicum (L.) quills pressurized water extracts

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dc.contributor.author Madushika Wariyapperuma, W.A.N.
dc.contributor.author Kannangara, S
dc.contributor.author Wijayasinghe, Y.S.
dc.contributor.author Subramanium, S
dc.date.accessioned 2021-10-22T06:56:44Z
dc.date.available 2021-10-22T06:56:44Z
dc.date.issued 2021
dc.identifier.citation Madushika Wariyapperuma WAN, Kannangara S, Wijayasinghe YS, Subramanium S, Jayawardena B. Fungal pretreatment to enhance the yield of phytochemicals and evaluation of α-amylase and α-glucosidase inhibition using Cinnamomum zeylanicum (L.) quills pressurized water extracts. Lett Appl Microbiol. 2021 Feb;72(2):196-205. doi: 10.1111/lam.13410. Epub 2020 Nov 10. PMID: 33030748. en_US
dc.identifier.uri http://repository.kln.ac.lk/handle/123456789/23294
dc.description.abstract Bioactive compounds entrapped in plant materials can be effectively recovered using fungal enzymes. Cinnamomum zeylanicum Sri Wijaya (SW) and Sri Gemunu (SG) accessions and commercially available C. zeylanicum (CC) were subjected to fungal pretreatment and extracted with pressured water (PWE, 0 098 MPa). Thirteen fungal species were isolated and the substrate utilization ability of the species was tested using cellulose, pectin and lignin (indirectly). Total phenolic content (TPC, Folin–Ciocalteu method), proanthocyanidin content (PC, vanillin method) and α-amylase and α-glucosidase inhibitory potential of the extracts were evaluated. The anti-diabetic drug, Acarbose was used as the positive control. Trichoderma harzianum (MH298760) showed the highest cell lysis ability and hence was used for the microbial pretreatment process. Extracts of SW treated with T. harzianum species (Pre-SW) gave the highest percentage yield (4 08% 0 15%), significantly potent inhibition (P < 0 05) of α-amylase and α-glucosidase activities (IC50 57 8 and 36 8 μg ml−1 respectively), TPC (2 24 0 02 mg gallic acid equivalent g−1), and PC (48 2 0 4 mg of catechin equivalent g−1) compared to Pre-SG, Pre- CC and nontreated samples. Trichoderma harzianum treatment can enhance the hypoglycaemic properties, PC and TPC of Cinnamon extracts and provide new insights into the recovery of phytochemicals. en_US
dc.publisher Lett Appl Microbiolgy en_US
dc.subject Cinnamomum zeylanicum, pressured water extraction, Trichoderma harzianum. α-amylase, α-glucosidase. en_US
dc.title Fungal pretreatment to enhance the yield of phytochemicals and evaluation of α-amylase and α- glucosidase inhibition using Cinnamomum zeylanicum (L.) quills pressurized water extracts en_US


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