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Potential of bio fabricated Annona glabra - silver nanoparticles to control Aedes aegypti and Aedes albopictus

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dc.contributor.author Aberathna, A.A.A.U.
dc.contributor.author Amarasinghe, L.D.
dc.contributor.author Sithara, W.S.
dc.contributor.author Wickramarachchi, S.
dc.contributor.author De Silva, C.R.
dc.date.accessioned 2020-10-20T19:06:36Z
dc.date.available 2020-10-20T19:06:36Z
dc.date.issued 2020
dc.identifier.citation Aberathna, A.A.A.U., Amarasinghe, L.D., Sithara, W.S., Wickramarachchi, S. and De Silva, C.R. (2020). Potential of bio fabricated Annona glabra - silver nanoparticles to control Aedes aegypti and Aedes albopictus, Proceedings of the 1st International Conference on Frontiers in Chemical Technology, Institute of Chemistry Ceylon, P.119 en_US
dc.identifier.uri http://repository.kln.ac.lk/handle/123456789/21436
dc.description.abstract Bio synthesized silver nanoparticles using Annona glabra leaf extract (An-AgNPs) as the reducing and stabrlizing agent were assessed as a novel larvicide against dengue vector mosquito larvae. The fresh leaves of A. glabra were crushed well and heated with distilled water under 70 0C for I hour. The supernatant was mixed (10% and 20% v/v of total volume) with 5 mM AgNO, to synthesize AnAgNPs. Formation ofAn-AgNPs were confirmed bythe color change and by appearance ofSPR peak in the Uv-vis spectrum and characterized by Dynamic Light Scattering (DLS), Scanning Electron Microscopy (SEM) and Fourier Transform Infrared spectroscopy (FTIR). lhe larvicidal activity of An-AgNPs was assessed against the third instar larvae of Ae. aegypti ^nd, Ae. albopictus, under the laboratory conditions (2 -10 mg/L). The change ofsolution color from yellow to dark brown/black and the SPR band at 435 nm confirmed the formation of An-AgNPs. According to SEM; An-AgNPs were spherical in morphology. FTIR analysis detected that An-AgNPs are capped and stabilized with biomolecules of A. glabraleaf ltZl9 (C=O stretching), 1366 (CH2 bending) and 3500-3200 (N-H and O-H stretching) and l2l7 cm-' (C-O stretching)i. The LCro of An-AgNPs (10%) and An-AgNPs (20%)againstthethirdinstarslarvaeofAe.aeglptiafter24hourswere5.2gand2.43mglland of Ae. albopictus were 3.02 and 2.51 mg/L respectively. The LC* of An-AgNPs (I0%) and An-AgNPs (20%)againstthethirdinstarslarvaeof Ae.aegyptiafter48hourswerel.5l andl.lT mg/Landof Ae. albopictus were 1.14 and 2.i0 mg/L respectively. The LC,n ofleafcrude plant extract against the third instars larvae of Ae. aegTpti after 24 and 48 hours were 5.9455 and 3.5485 mg/L and for the third instars iaruae of Ae. albopictus were 5.0040 arrd2.7346 mg/L respectivdy. This study shows that AnAgNPs is efficient in controlling mosquito larvae. en_US
dc.language.iso en en_US
dc.publisher , Proceedings of the 1st International Conference on Frontiers in Chemical Technology, Institute of Chemistry Ceylon en_US
dc.subject A. aegypti, A. albopictus, A. glabra, Larvicidal activity, Ag nanoparticles en_US
dc.title Potential of bio fabricated Annona glabra - silver nanoparticles to control Aedes aegypti and Aedes albopictus en_US
dc.type Article en_US


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