Digital Repository

Screening of beach sand for bacterial treasures - A preliminary approach.

Show simple item record

dc.contributor.author Joseph, T. T.
dc.contributor.author Abeygunawardena, S. I.
dc.date.accessioned 2017-11-21T08:30:14Z
dc.date.available 2017-11-21T08:30:14Z
dc.date.issued 2017
dc.identifier.citation Joseph, T. T. and Abeygunawardena, S. I. (2017). Screening of beach sand for bacterial treasures - A preliminary approach. International Research Symposium on Pure and Applied Sciences, 2017 Faculty of Science, University of Kelaniya, Sri Lanka.p45. en_US
dc.identifier.uri http://repository.kln.ac.lk/handle/123456789/18179
dc.description.abstract Marine microorganisms are of great interest as novel and rich sources of biologically active products. The micro-organisms living in both sea water and sand must be able to survive and grow under harsh conditions. Due to these adaptations, the metabolites produced by marine micro-organisms possess unique features and they serve as promising choices for many different biotechnological applications. The aforesaid micro-organisms are referred to as ‘microbial treasures’ at present. In this study, preliminary screening for different culturable bacteria present in shoreline sand was carried out and tested for different biological activities. Morphologically different bacterial isolates were identified using the standard morphological and biochemical characterization procedures in microbiology. The results indicated that the majority of the isolates belong to the genus Bacillus and the others were the species of Micrococcus, Aerococcus, Pseudomonas, and Staphylococcus. The degradation of two dyes, crystal violet and saffranin were tested and the degradation was only observed for crystal violet with two Bacillus isolates i.e. SSMA-15 and SSPC-16. Some isolated bacteria accumulated the dyes in their cells. The highest indole acetic acid (IAA) production was observed for the Bacillus-SSPC-11. Antagonistic effect against all three selected bacteria, i.e. S. aureus, B. subtilis and E. coli was only shown by the Pseudomonas-SSMA-7. Along with the antagonistic effects, it also produced the protease and cellulase enzymes. The bacterial isolate-SSMA-9 belonging to the family Enterobacteriaceae exhibited the highest number of positive activities during this study. It included the production of cellulase, laccase, amylase and protease enzymes and the accumulation of the saffranin and crystal violet dyes. The Bacillus-SSPC-3 showed positive production of three enzymes; amylase, protease and cellulase. Staphylococcus-SSPC-12 has the ability to accumulate the two dyes in addition to the production of the two enzymes amylase and protease. Based on the positive activities and produced components, bacterial isolates SSMA-9, SSMA-7 and SSPC-12 were identified as possible bacterial treasures. en_US
dc.language.iso en en_US
dc.publisher International Research Symposium on Pure and Applied Sciences, 2017 Faculty of Science, University of Kelaniya, Sri Lanka. en_US
dc.subject Bacterial treasures en_US
dc.subject Cellulase en_US
dc.subject Indole acetic acid en_US
dc.subject Sea sand en_US
dc.title Screening of beach sand for bacterial treasures - A preliminary approach. en_US
dc.type Article en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search Digital Repository


Browse

My Account