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Secondary Metabolites with Radical Scavenging Activity from Daldinia Eschcholzii, Isolated from The Lichen Parmotrema Sp. In Sri Lanka-Isolation and Structure Determination

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dc.contributor.author Manthrirathna, M.A.T.P.
dc.contributor.author Kandiah, R.
dc.contributor.author Gunasekara, D.S.
dc.contributor.author Paranagama, P.A.
dc.date.accessioned 2017-01-27T03:44:58Z
dc.date.available 2017-01-27T03:44:58Z
dc.date.issued 2016
dc.identifier.citation Manthrirathna, M.A.T.P., Kandiah, R., Gunasekara, D.S. and Paranagama, P.A. 2016. Secondary Metabolites with Radical Scavenging Activity from Daldinia Eschcholzii, Isolated from The Lichen Parmotrema Sp. In Sri Lanka-Isolation and Structure Determination. In proceedings of the 17th Conference on Postgraduate Research, International Postgraduate Research Conference 2016, Faculty of Graduate Studies, University of Kelaniya, Sri Lanka. p 163. en_US
dc.identifier.uri http://repository.kln.ac.lk/handle/123456789/16066
dc.description.abstract Natural products are promising leads for novel therapeutic agents. Isolation and characterization of bioactive compounds in search for potential pharmocophores has acquired a developing interest in on-going research. Although Endolichenic fungi (EF) are a rich source of bioactive secondary metabolites, they still remain almost unexploited. The present study is focused on isolation and structure elucidation of compounds with radical scavenging activity from the EF, Daldinia eschscholzii that occur in the lichen Parmotrema sp. in Hakgala Botanical Garden, Sri Lanka. Daldinia eschscholzii cultivated on 48 large petri dishes with PDA were incubated at room temperature for one week. Mycelia were cut in to small pieces along with the medium and extracted with ethyl acetate twice. The radical scavenging activity of the crude extract was evaluated using DPPH assay. Standard antioxidant, Butylated Hydroxy Toluene (BHT) and MeOH were used as the positive control (IC50= 38.2 ± 4.0 μg/ mL) and negative control respectively. The crude extract with high radical scavenging activity (IC50 = 77.9 ± 5.1 μg/ mL), was partitioned with hexane, chloroform and aqueous methanol. All three organic extracts were then subjected to DPPH assay. Chloroform fraction with the highest activity (IC50= 63.8 ± 4.8 μg/ mL) was further fractionated using silica gel, sephadex column chromatography and preparative TLC to isolate two pure compounds. The structures of the compounds were elucidated using 1H, 13C, 2D NMR and MS data. The compounds were identified as 7-hydroxy- 2-methylchroman-4-one (1) and 5-methoxynaphthalen-1-ol (2). Compound 1 showed no activity in the assay. Compound 2 showed higher activity than the standard BHT, with IC50 value of 10.2 ± 5.8 μg/ mL. en_US
dc.language.iso en en_US
dc.publisher Faculty of Graduate Studies, University of Kelaniya, Sri Lanka en_US
dc.subject Daldinia eschscholzii en_US
dc.subject endolichenic fungi en_US
dc.subject radical scavenging activity en_US
dc.subject secondary metabolites en_US
dc.subject DPPH assay en_US
dc.title Secondary Metabolites with Radical Scavenging Activity from Daldinia Eschcholzii, Isolated from The Lichen Parmotrema Sp. In Sri Lanka-Isolation and Structure Determination en_US
dc.type Article en_US


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