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Study of pollen germination of Dendrobium cultivars

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dc.contributor.author Silva, W.E.R.
dc.contributor.author Kahagalla, T.H.
dc.contributor.author Senanayake, S.P.
dc.contributor.author Attanayake, R.
dc.date.accessioned 2016-12-28T09:23:06Z
dc.date.available 2016-12-28T09:23:06Z
dc.date.issued 2016
dc.identifier.citation Silva, W.E.R., Kahagalla, T.H., Senanayake, S.P. and Attanayake, R. 2016. Study of pollen germination of Dendrobium cultivars. In Proceedings of the International Research Symposium on Pure and Applied Sciences (IRSPAS 2016), Faculty of Science, University of Kelaniya, Sri Lanka. p 15. en_US
dc.identifier.isbn 978-955-704-008-0
dc.identifier.uri http://repository.kln.ac.lk/handle/123456789/15667
dc.description.abstract Orchidaceae family includes more than 25,000 species, among which Dendrobium genus is one of the most numerous with over 1,200 species in the world. Orchid pollen is packaged as pollinia in which the pollen grains are bound together by viscin threads in masses for effective pollination. Palynological studies of Dendrobium are more important as orchid pollen cannot be dispersed by wind and carried to the stigma for effective fertilization but is mostly done by insects and human beings (hand pollination). Thus, this study was aimed at comparing the efficiency of in vitro pollen germination and natural pollen germination. In vitro pollen germination of commercial Dendrobium variety ‘Thailand tommy’and ‘Burania jet green’ was evaluated using three liquid media containing sucrose (0, 5%, 10%, 15%, 20%) with 0.01% boric acid medium, BK medium containing sucrose, H3B03 (100 mg/L), Ca(N03)2 (300 mg/L), MgSO4 (200 mg/L), and KNO3 (100 mg/ L) and sucrose solutions. Pollen were collected from newly opened flowers of two Dendrobium cultivars, sown on different media and then incubated at room temperature for 24h and examined under a light microscope (10 x 40). Natural pollen germination on stigmatic fluid of Dendrobium cultivars was evaluated using hand pollinated Dendrobium flowers. The pollinia of Dendrobium cultivars were deposited on stigma of newly opened flowers and after 72 hours the small amount of stigmatic fluid was stained with cotton blue in lacto phenol and observed under a light microscope (10 x 40). Pollen tube elongation equal or greater than the diameter of the pollen was considered as germinated pollen. Highest percentage of mean pollen germination was recorded in 0.01% boric acid containing 20% sucrose medium for both ‘Thailand tommy’ (16%) and ‘Burania jet green’ (12.50%) while the lowest mean germination was seen in distilled water medium in ‘Thailand tommy’ (2%) and ‘Burania Jet Green’ (1.50%) in the in vitro pollen germination process. In natural pollen germination process, the percentage of mean pollen germination on stigmatic fluid of Dendrobium cultivars was 26.6% and the pollen tube was elongated 5 times than the pollen diameter. en_US
dc.language.iso en en_US
dc.publisher Faculty of Science, University of Kelaniya, Sri Lanka en_US
dc.subject Dendrobium cultivars en_US
dc.subject Pollen germination en_US
dc.subject Pollinia en_US
dc.title Study of pollen germination of Dendrobium cultivars en_US
dc.type Article en_US


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