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Evaluation of the ICT whole-blood antigen card test to detect infection due to Wuchereria bancrofti in Sri Lanka

Show simple item record Chandrasena, T.G.A.N. en_US Premaratna, R. en_US Abeyewickreme, W. en_US de Silva, N.R. en_US 2014-10-29T09:19:22Z 2014-10-29T09:19:22Z 2002 en_US
dc.identifier.citation Transactions of the Royal Society of Tropical Medicine and Hygiene. 2002; 96(1): pp.60-63 en_US
dc.identifier.issn 0035-9203 (Print) en_US
dc.identifier.issn 1878-3503 (Electronic) en_US
dc.description Indexed in MEDLINE
dc.description.abstract The sensitivity, specificity and cost effectiveness of an immunochromatographic card test (ICT, AMRAD) for the diagnosis of bancroftian filariasis were estimated against 2 standard parasitological techniques: thick blood film (TBF) and Nuclepore membrane filtration (NMF). Individuals were selected from endemic localities in the Western Province (n = 213) and from the non-endemic Central Province (n = 29) of Sri Lanka. Blood was collected between 21:00 and midnight. Sixty microlitre of non-heparinized blood, and 1 mL and 100 microL of heparinized blood were used in TBF, NMF andICT, respectively. NMF was positive in 31.5% (67/213) of the endemic group, with a mean microfilaria (mf) count of 343/mL (range 8-1782, SD 422). All 67 were positive by ICT (sensitivity 100%), but only 63 by TBF (sensitivity 94%). Among the endemic population there were 12 who were mf negative but antigen positive by ICT. There were, however, no false positives among the non-endemic controls, indicating the possibility that the ICTmay in fact be more sensitive and 100% specific. Thus, ICT filariasis test appears to be more effective (both sensitive and specific) than TBF or NMF in diagnosing infection in lymphatic filariasis. The direct unit recurrent costs of the 2 survey tools, TBF and ICT, were US$ 0.30 (Rs. 27/=) and US$ 2.75 (Rs. 248/=), respectively. The high cost of the ICT may be offset by other factors that are difficult to cost. en_US
dc.publisher Oxford University Press en_US
dc.subject Filariasis en_US
dc.subject Clinical Trial en_US
dc.subject Comparative Study en_US
dc.subject Controlled Clinical Trial en_US
dc.subject Antigens, Helminth-blood en_US
dc.subject Cost-Benefit Analysis en_US
dc.subject Filariasis-diagnosis en_US
dc.subject Reagent Kits, Diagnostic-standards en_US
dc.subject Wuchereria bancrofti-isolation and purification en_US
dc.title Evaluation of the ICT whole-blood antigen card test to detect infection due to Wuchereria bancrofti in Sri Lanka en_US
dc.type Article en_US
dc.identifier.department Parasitology en_US
dc.identifier.department Medicine en_US
dc.creator.corporateauthor Royal Society of Tropical Medicine and Hygiene en_US

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