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Application of a real time Polymerase Chain Reaction (PCR) assay for the early diagnosis of human leptospirosis in Sri Lanka

Show simple item record Denipitiya, D.T.H. en_US Chandrasekharan, N.V. en_US Abeyewickreme, W. en_US Hartskeerl, C.M. en_US Hartskeerl, R.A. en_US Jiffrey, A.M. en_US Hapugoda, M.D. en_US 2016-10-28T07:24:30Z en_US 2016-10-28T07:24:30Z en_US 2016 en_US
dc.identifier.citation Biologicals. 2016; 44(6):497-502. en_US
dc.identifier.issn 1045-1056 (Print) en_US
dc.identifier.issn 1095-8320 (Electronic) en_US
dc.identifier.issn 1045-1056 (Linking) en_US
dc.identifier.uri en_US
dc.description Indexed in MEDLINE en_US
dc.description.abstract Leptospirosis has a major impact on health in Sri Lanka but is probably grossly under-recognized due to difficulties in clinical diagnosis and lack of diagnostic laboratory services. The objective of this study was to establish and evaluate a SYBR Green-based real-time Polymerase Chain Reaction (rt-PCR) assay for early, rapid and definitive laboratory diagnosis of leptospirosis in Sri Lanka. The rt-PCR assay was established and analytical specificity and sensitivity were determined using reference DNA samples. Evaluation of the assay for diagnosis of clinical samples was performed using two panels of serum samples obtained from 111 clinically suspected adult patients. Patients were confirmed as leptospirosis (n = 65) and non-leptospirosis (n = 30) by the Patoc - MAT. Other 16 samples gave ambiguous results. The analytical sensitivity of the rt-PCR was approximately 60 genome copies and no cross-reactivity was observed with saprophytic Leptospira spp. and other pathogenic microorganisms. Based on confirmation with Patoc-MAT on paired samples this corresponds to a diagnostic sensitivity and specificity of 67.7% (44/65) and 90.0% (27/30), respectively. This study showed that rt-PCR has the potential to facilitate rapid and definitive diagnosis of leptospirosis during early phase of infection in Sri Lanka. en_US
dc.language.iso en_US en_US
dc.publisher Academic Press, Elsevier en_US
dc.subject Leptospirosis en_US
dc.subject.mesh Leptospirosis-diagnosis en_US
dc.subject.mesh Leptospirosis-blood en_US
dc.subject.mesh Leptospirosis-genetics en_US
dc.subject.mesh Leptospira-genetics en_US
dc.subject.mesh DNA, Bacterial-blood en_US
dc.subject.mesh DNA, Bacterial-genetics en_US
dc.subject.mesh Real-Time Polymerase Chain Reaction en_US
dc.subject.mesh Real-Time Polymerase Chain Reaction- methods en_US
dc.subject.mesh Sri Lanka en_US
dc.subject.mesh Clinical Trial en_US
dc.title Application of a real time Polymerase Chain Reaction (PCR) assay for the early diagnosis of human leptospirosis in Sri Lanka en_US
dc.type Article en_US

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