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Characterization of the Temperate Phage vB_RleM_PPF1 and its Site-Specific Integration into the Genome of Rhizobium leguminosarum Strain F1

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dc.contributor.author Halmillawewa, A.P.
dc.contributor.author Restrepo-Córdoba, M.
dc.contributor.author Perry, B.J.
dc.contributor.author Yost, C.K.
dc.contributor.author Hynes, M.F.
dc.date.accessioned 2015-12-16T08:45:45Z
dc.date.available 2015-12-16T08:45:45Z
dc.date.issued 2015
dc.identifier.citation Halmillawewa, A.P., Restrepo-Córdoba, Marcela, Perry, Benjamin J., Yost, Christopher K. and Hynes, Michael F. (2015). Characterization of the Temperate Phage vB_RleM_PPF1 and its Site-Specific Integration into the Genome of Rhizobium leguminosarum Strain F1. In: Research Forum E Proceeding, Staff Development Centre Research Forum, Cycle 14-2015, University of Kelaniya, Kelaniya, pp 02-03.
dc.identifier.issn 2448-9743
dc.identifier.uri
dc.identifier.uri http://repository.kln.ac.lk/handle/123456789/10832
dc.description.abstract The presence of prophages in a genome can contribute in increasing the bacterial fitness and ecological success in an environment that contains closely related phages. The temperate phage PPF1 was isolated from a lysogenized strain of Rhizobium leguminosarum F1. The complete genome sequence of Myoviridae phage PPF1 was determined using 454-Pyrosequencing technology. PPF1 is the first available complete genome sequence of a Rhizobium leguminosarum temperate phage (GenBank accession no: KJ746502) and the integration site and possible mechanism of integration of this phage has been identified. PPF1 is capable of efficiently lysogenizing the R. leguminosarum strain F1, and can be induced from its lysogenized host using UV irradiation. The genome of PPF1 is 54,506 bp in length with an average G+C content of 61.9%. The ORF predictions of the PPF1 genome revealed the presence of 94 putative proteinencoding genes and 74.5% of these predicted ORFs share homology at the protein level with previously reported sequences in the database. However, putative functions could only be assigned to 25.5% (24 ORFs) of the predicted genes. The site-specific recombination system of the phage targets an integration site that lies within a putative tRNA-Pro (CGG) gene in R. leguminosarum F1. Upon integration, the phage is capable of restoring the disrupted tRNA gene, owing to the 50 bp homologous sequence (att core region) it shares with its rhizobial host genome. The predicted att site of temperate phage PPF1 share a sequence similarity with the targeted att site of previously characterized Sinorhizobium meliloti phage 16-3. In spite of phage PPF1's propensity for lysogenizing host strains, including strains on which it does not form visible plaques, there has thus far been no evidence of the presence of similar phages integrated into completed genomes of R. leguminosarum and related phages. The site specificity of insertion of this phage could be used to create single copy integration vectors for genetic work in R. leguminosarum. en_US
dc.language.iso en en_US
dc.publisher Staff Development Center, University of Kelaniya en_US
dc.title Characterization of the Temperate Phage vB_RleM_PPF1 and its Site-Specific Integration into the Genome of Rhizobium leguminosarum Strain F1 en_US
dc.type Article en_US


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