International Postgraduate Research Conference (IPRC)

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Subject: search.filters.subject.Antioxidant activity

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    Nutritional Effect of Consumption of Domestic and Commercially Available Coconut Milk Preparations in Wistar Rats.
    (In: Proceedings of the International Postgraduate Research Conference 2017 (IPRC – 2017), Faculty of Graduate Studies, University of Kelaniya, Sri Lanka., 2017) Senanayake, C.M.; Seneviratne, K.N.; Jayathilaka, N.; Ekanayaka, S.
    The use of both domestic coconut milk (CM) preparations and commerciallyavailable CM preparations in cooking has become popular. The present study involves evaluating In vivo effect of domestic CM prepared by blending (BCM), commercially available powdered CM (PCM) and liquid CM (LCM) on serum lipid profiles and serum antioxidant capacity using Wistar rats. Seven weeks old male Wistar rats were randomly assigned into treatment groups. Control group was fed with a semisynthetic diet recommended by WHO. Second, third and fourth groups were fed with semi synthetic diet which contains 12 mL BCM, PCM or LCM per kg of feed respectively. Blood was drawn on day before feeding experimental diets (Day 0), 30 days, 90 days, 120 days and 150 days after feeding experimental diets. Serum total cholesterol (TC), high density lipoprotein (HDL) and triglycerides (TG) were analyzed using a test kit. Low density lipoprotein (LDL) was determined using Friedewald equation. Antioxidant activity of serum was determined by ABTS assay and DPPH radical scavenging assay. TC levels of all groups were significantly (p<0.05) increased after 150 days of feeding compared to their day 0 levels. TC levels (mg/dL) of rats fed with BCM (80±4), PCM (80±5) and LCM (81±3) were similar to control group (77±7). However, rats fed with LCM showed a statistically significant (p<0.05) increase in TC compared to control group. Although, TG levels of CM diet groups indicated significant (p<0.05) increase on day 150 compared to their day 0 levels, these levels were similar to that of control group. Both HDL and LDL levels of CM diet groups remained same compared to control group at day 150. All CM diet groups showed a significantly (p<0.05) increased activity on day 150 compared to their day 0 levels.CM fat contains nearly 90 % saturated fat. However, majority of the saturated fat in CM fatis composed of short and medium chain fatty acids, which are beneficial to health. As such, adding CM to diet did not affect average levels of serum TC, LDL and TG of Wistar rats suggesting that none of the CM preparations under investigation contribute to detrimental changes in lipid profiles. All CM preparations, on the other hand, appear to increase serum antioxidant activity which may contribute in retarding oxidative damage to biomolecules. Financial assistance from National Research Council (12-012) and University Research Grant (RP/03/02/06/05/2015) areacknowledged.
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    Bioactive compounds and in-vitro antioxidant potential of mango (Mangifera indica L.) flesh, peel and seed
    (Faculty of Graduate Studies, University of Kelaniya, 2015) Thiripuranathar, K.G.; Navaratne, A.N.; Paranagama, P.A.
    Natural antioxidant compounds from flesh, peel and seed of fruits have gained increasing attention in the scientific community. Synthetic antioxidants cause the side effects such as cancer and cardiovascular diseases. The antioxidants from natural sources are the only alternative to synthetic antioxidant. The main objective of this study was to evaluate the potential efficacy of mango peel and seed kernel as an antioxidant.In the present study three mango varieties ‗Willard‘, ‗Karuthacolomban‘ and ‗Vellaicolomban‘ were collected from Jaffna district during April to July, 2015. Ethyl acetate extracts of flesh, peel and seed kernel of three mango varieties were studied for the total phenolic content (TPC) and total flavonoid content (TFC) using Folin-Ciocalteu method and aluminium chloride colorimetric assay respectively. A study of in vitro antioxidant activities were also studied using different assays, which include such DPPH radical scavenging assay, Ferric ion reducing power assay (FRAP), Nitric oxide scavenging assay (NO.) and ABTS radical scavenging assay(ABTS.+).The results revealed that peel of Willard showed the highest TPC (275.61±5.24 mg GAE /g extract) and flesh of Vellaicolomban showed the highest TFC (479.8015.30 mg QE/g extract). The DPPH radical scavenging activity results revealed that the ethyl acetate extract of Karuthacolomban seed kernel (IC50-7.7±0.4 μg/mL) showed a higher radical scavenging activity than that of the standard, ascorbic acid (IC50- 11.0±0.4μg/mL). Peel obtained from Willard and seed kernel obtained from Karuthacolomban showed the highest antioxidant activity in FRAP assay. The NO. assay results indicated that seed kernel of Karuthacolomban(IC50-60.6±3.5μg/mL) showed significantly higher scavenging activity than other eight extracts, but it showed lower activity than, ascorbic acid(IC50-22.3±1.6 μg/mL).The ABTS.+ assay indicates that seed kernel of Kruthacolomban (IC50-46.4±1.9 μg/mL) showed higher radical scavenging activity than that of the standard, Trolax (IC50-136.6± 5.2μg/mL). Finding of the present investigation showed that the mango peel and seed kernel contained more polyphenols and exhibited good antioxidant activity than that of flesh.