International Postgraduate Research Conference (IPRC)

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    Evaluating the Antibiotic Properties of Bee Honey as Against Common Pathogenic and Antibiotic Resistant Bacteria Found in Wound Infections
    (International Postgraduate Research Conference 2019, Faculty of Graduate Studies, University of Kelaniya, Sri Lanka, 2019) Wijesooriya, L.I.; Abeysundara, S.
    Introduction: Antibiotics have largely been effective in treating bacterial infections. However, inappropriate use of antibiotics has led to extensive antibiotic resistance globally. In addition, no new classes of antibiotics are available to counter the dramatic rise of antibiotic resistance. This has led to unorthodox methods in treating antibiotic-resistant bacterial infections. One of these methods is the use of bee honey, which has been used since ancient times. Objective: The objective of the present study was to determine the effectiveness of bee honey against common pathogenic bacteria in wound infections Methodology: Common bacterial wound pathogens viz. Streptococcus pyogenes, Streptococcus agalactiae, methicillin resistant Staphylococcus aureus (MRSA), Escherichia coli (extended-spectrum beta-lactamases producing), Klebsiella pneumoniae, Acinetobacter spp, and Enterococcus spp were selected for the study. Suspensions of 0.5 McFarland strength of S. pyogenes was streaked on blood agar and the others were streaked on Muller Hinton agar. Sterile, 1"x1" size, single gauze layer, soaked with commercially available pure Bee honey (100%) was placed on the center of each inoculated plate and incubated overnight at 37 oC. On following day, each piece of gauze was removed aseptically. Presence of live bacteria from the site where gauze was removed from each plate was checked by streaking on blood agar. The same place where gauze was removed was repositioned with new, sterile gauze layer soaked with bee honey. All plates were incubated at 37 oC. Same procedure was continued until no growth was observed. Control tests were done in parallel using same sized gauze without bee honey. Sterility of bee honey was previously confirmed by inoculation on blood agar. The experiment was repeated two times. Results: Following repeat applications of bee honey, there was no bacterial growth from plates inoculated with S. pyogenes and S. agalactiae after two applications, Acinetobacter spp - three, MRSA – four, K. pneumoniae, E.coli and Enterococcus spp - five and Pseudomonas spp – six applications. There was no inhibition of bacterial growth in the control plates. Conclusion: Antibacterial effect of bee honey against S. pyogenes and S. agalactiae was highly satisfactory with clearance of the organisms with only two applications whereas it was satisfactory against MRSA, Acinetobacter spp. Klebsiella spp, E.coli and Enterococcus spp and longest duration of application was needed for Pseudomonas spp. These in vitro anti-bacterial test results suggest that bee honey has effective antibacterial property against common wound pathogens with varying duration of application
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    Antibacterial activity of extract of leaves of Atalantia ceylanica (Yakinaran)
    (Faculty of Graduate Studies, University of Kelaniya, 2015) Munasinghe, D.A.L.; Karunarathna, E.D.C.; Sudesh, A.D.H.
    Antibacterial activity of leaves of Atalantia ceylanica was tested qualitatively against 05 bacterial strains with the modified plate method at Gampaha Wickramarachchi Ayurveda Institute. The steam of boiled leaves and extract are used by certain villagers as a traditional treatment for healing respiratory ailments such as influenza. Even though natural flora may be effective in curing health defects, scientific studies on subject is limited. Thus the study was arranged to fill the gap for certain extent to harness natural resources effectively in health. So as the study was aimed to investigate the antibacterial properties of Atlanta leaves. The plates, prepared with the watery extract of boiled leaves, which comprised of neat concentration of raw leaf materials and Mueller-Hinton agar were inoculated with laboratory stored standard bacterial strains including Pseudomonas aeruginosa, Streptococcus pneumonia, Escherichia coli, Staphylococcus aureus and Methicillin resistant Staphylococcus aureus separately, which can cause respiratory as well as gastro- intestinal symptoms. For the control test, distilled water was used to make plates instead of watery leaf extract. Even after the duplicated experiment, no strain was found to be inhibited by the leaf materials, which indicated that the neat concentration of raw chemical compound of particular leaves have no inhibitory action on tested strains. As the respiratory infection is not only caused by bacteria but also by viruses, perhaps phytochemicals might have possessed antiviral as well as antibacterial properties on organisms other than the tested strains. Occasionally the chemicals of the particular plant leaves might have possessed certain antiinflammatory features to control respiratory symptoms. Nevertheless it was clear that the chemical compounds of the distillation of leaves of Atalantia ceylanica had no significant inhibitory effect on the growth of tested strains. The same experiment was carried out early for the steam of particular leaf and the results were found to be the same.