International Postgraduate Research Conference (IPRC)

Permanent URI for this communityhttp://repository.kln.ac.lk/handle/123456789/155

Browse

Has files: YesSubject: search.filters.subject.amylase

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Production of Amylase by A. Niger Under Submerged Fermentation Using Pineapple Peel as The Substrate and Its Application in Baking Industry
    (Faculty of Graduate Studies, University of Kelaniya, Sri Lanka, 2016) Pushpakumara, H.M.P.J.; Weerasooriya, M.K.B.
    Starch-degrading amylase was produced by A. niger under submerged condition utilizing pineapple peels as the substrate. Growth parameters were optimized by changing incubation period, pH of the culture media, level of carbon source (dried pineapple peel powder) and additional nitrogen source, in order to get maximum amylase production. Study revealed that the production of extracellular amylase increased with the culture growth and reached to maximum at day 7 in the cultures grown at pH 5.0. Studies with different levels of carbon source indicated that optimum level for carbon source for maximum amylase production was 18 gL-1. Effect of additional nitrogen source on amylase production was also monitored supplementing the growth media with different nitrogen sources such as peptone, gelatin and urea. The result indicates that using gelatin as additional nitrogen source increase the amylase production. Experiments were also carried out to monitor extracellular and intracellular amylase production and the results revealed that extracellular amylase production was found to be higher than that of intracellular. Yield of the enzyme was 8530 units/g of pine apple peel powder. To test suitability of the enzyme for improvement of the quality of bakery products bakery products were prepared by treating the wheat flour dough with different volumes of partially purified enzyme Results showed that Treating 7.7 mL (11.5 UmL-1) partially purified amylase with one kg of wheat flour dough provide better quality product with improved shiny appearance, color, crumb structure, taste and better anti-staling effect than the control.
  • Thumbnail Image
    Item
    In Vitro Inhibitory Potentials of Alpha Amylase and Alpha Glucosidase in Tricosanthes Dioica, Moringa Oleifera And Averrhoa Carambola
    (Faculty of Graduate Studies, University of Kelaniya, 2015) Jayawardena, B.; Ranasinghe, M.
    In recent times type 2 diabetes has become one of the major public health concern in both developing and developed countries. It is defined as an endocrine and metabolic disease defined by elevated blood glucose level (hyperglycemia). Current studies based on medicinal plants have revealed that phenolic and flavonoids compounds present in plants can inhibit key enzymes such as alpha glucosidase and alpha amylase and offer an effective strategy to control post prandial hyperglycaemia. Therefore the purpose of this study was to determine the inhibitory potential of alpha glucosidase and alpha amylase byTricosanthesdioica (Padwal), Moringaoleifera (Murunga)and Averrhoacarambola (Kamaranga). Leaves of Padwal, Murunga and fruits of Kamaranga were sliced, dried, powdered and refluxed with 75% ethanol and fractionated with ethyl acetate. 75% ethanol extract, ethyl acetate extract and aqueous layers were obtained. Each layer was tested for the total phenolic content and percentage reducing activity. IC50 values of alpha amylase and alpha glucosidase inhibitions by the extractswere determined using several biochemical assays. Among the three plantsT. dioica 75% ethanol extract had the highest amount of phenolic content (50.88±4.90 mg/g) and the highest reducing power was in A. carambola 75% ethanol extract (710.91±15.62). The most plant extracts showed higher reducing power compared with standard BHT (317.27±19.10). In the determination of IC50 values for alpha amylase, ethyl acetate layer of A. carambola (57.36 ± 16.51 μg/g) showed the lowest value and the highest IC50value was inT. dioica aqueous layer (1225.84±59.57 μg/g). The lowest IC50 value for alpha glucosidasewas observed in M. oleifera (279.84±35.51 ng/g) aqueous layer and the highest value was in the ethyl acetate extract of T. dioica(9.08×105±1.11×104ng/g). In the present study the selected plant materials had high phenolic contents and percentage reducing power. A. carambola ethyl acetate extract is most effective inhibitor for amylase and M. oleifera aqueous layer is effectual inhibitor for glucosidase in vitro among the three plants.