Botany

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    Microbial Status of Fresh Cut Cooking Banana Variety Alukesel (Musa acuminata× Musa balbisiana, ABB Group) as Affected by Pre-treatments
    (2016) Siriwardana, H.; Abeywickrama, K.P.; Herath, I.
    Fresh cut (minimally processed) cooking banana variety Alukesel was subjected to different pretreatments, packed in polystyrene packages and stored at 5-7 °C for a week. Effect of several identified pre-treatments on Total Plate Count (TPC) and Total Yeast and Mould counts (TYM) were evaluated. Bacteria isolated from plates were identified using biochemical tests and molecular tools. In pre-treated samples, microbial counts were within safe-to-consume limits. Bacillus cereus, Enterobacter ludwigii and Bacillus thuringiensis were identified from fresh cut samples using molecular tools. Citric acid at 3% w/v effectively controlled bacteria, yeasts and moulds and this observation was significantly different from the control (p<0.05). The present study has shown that 3% citric acid was the most effective pretreatment for minimal processing of Alukesel which controlled bacteria, yeasts & moulds completely. Alukesel pretreated with 3% citric acid was free from food borne pathogens such as Salmonella, Clostridium, Yersinia, and Listeria.
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    Quality assessment minimally processed immature jackfruit (Artocarpus heterophyllus, Lam.) as affected by pretreatments
    (Association of Food Scientists & Technologists, India, 2015) Ekanayaka, H.; Abeywickrama, K.P.; Jayakody, R.; Herath, I.
    Abstract Degree of browning, firmness, sensory properties and microbiological quality of minimally processed immature jakfruit were investigated on initial and seventh day after stor- age at 5–7 °C. Samples were pretreated with 0.5 % and 1 % sodium metabisulphite, 1.5 % citric acid+1.5 % ascorbic acid, 3 % citric acid, 3 % ascorbic acid and packed in polystyrene packages and over wrapped with polyvinylchloride (PVC) stretch film before storage. When degree of browning in rela- tion to absorbance was assessed, immature jakfruit pretreated with 1.5 % citric acid+1.5 % ascorbic acid showed the lowest absorbance at 420 nm among all pretreated samples indicating low browning in samples. At 450 nm, minimally processed immature jackfruit samples pretreated with 1.5 % citric acid+ 1.5 % ascorbic acid showed the highest reflectance (indicating lowest absorbance) compared to other treatments. Variations in firmness were observed after treatment. Microbial counts were within safe-to-consume limits while coliforms were not detected in any of the samples. Erwinia aphidicola and Bacillus subtilis were identified from minimally processed immature jakfruit samples using molecular tools. 1.5 % citric acid+1.5 % ascorbic acid, 1 % sodium metabisulphite and 3 % citric acid were relatively more successful in retaining acceptable sensory quality of jakfruit even after 7 days of storage. Above pretreatments could be recommended during minimal processing of immature jakfruit meant for local sale in supermarkets where cold storage is available.