Browsing by Author "Waduge, V."

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    Accumulation of toxic metals in ectopic endometrial tissue
    (Sri Lanka Medical Association, 2011) Silva, N.; Senanayaka, H.; Peiris-John, R.; Wickremasinghe, R.; Waduge, V.
    INTRODUCTION AND OBJECTIVES: Aim of this study was to determine the association between heavy metals and endometriosis. METHODS: A case-control study was conducted in a tertiary care hospital comparing 50 cases of endometriosis with 50 age matched controls confirmed by laparoscopy or laparotomy. Blood samples and ectopic endometrial tissue samples were obtained and digested with supra pure 65% HNO3. Samples were analysed for cadmium by graphite furnace atomic absorption spectroscopy (GFASS). In a subset of cases (n=30) and controls (n=40) samples were analyzed for nickel, lead and zinc by Total Reflection X-ray Fluorescence (TXRF) technique. T-tests and Spearman's correlation coefficient were used for analysis. RESULTS: Cases had significantly higher (p=0.011) mean (+SD) blood nickel levels (1.69+1.18 ug/1) as compared to controls (0.96+0.94 ug/1). Blood levels of cadmium (0.75±0.46 ug/I vs 0.77 ±0.37 ug/1) lead (5.8S±1.25 ug/1 vs 3.45±1.25 ug/1) and zinc (123.02±2.49 ug/1 vs151.35 ±2.46 ug/1) were similar in the two groups (p=0.289, p=0.123s and p=0.339, respectively). Tissue samples had significantly higher (p=0.001, p<0.001, p=0.049 respectively) mean (+SD) levels of cadmium (2.86±0.61 ug/1 vs 0.8±0.48 ug/1) nickel (8.64±0.4.12 ug/Lvsl.58±1.21ug/l) and lead (11.22±4.67 ug/1 vs 5.86±3.38 ug/1) compared to blood levels. Mean (+SD) blood levels of zinc (117.48 ±2.64 ug/1) was higher (p<0.001) than that in tissues (27.54±6.65 ug/1). There was a positive correlation between blood zinc levels and tissue zinc levels (r=0.417;p= 0.034) but there was no correlation between blood and tissue levels of cadmium, nickel and lead (r=0.237,p=0.085; r=0.372,^=0.113; r=0.70,/?=0.067, respectively). CONCLUSIONS: Cadmium, nickel and lead but not zinc accumulate in ectopic endometrial tissue.
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    Association between heavy metals and endometriosis
    (Sri Lanka Medical Association, 2010) Silva, N.L.; Senanayaka, H.; Peiris-John, R.; Wickremasinghe, R.; Waduge, V.
    OBJECTIVE: To determine the association between heavy metal concentrations in whole blood and ectopic endometrial tissue in endometriosis. METHODS: A case-control study was conducted in a tertiary care hospital. Endometriosis was confirmed by laparoscopy or laparotomy in 14 patients, There were 11 age-matched controls with no evidence of the disease. Informed written consent was obtained pre-operatively. Blood samples were collected from all participants. In 10 patients ectopic endometrial tissue samples were obtained by a single surgeon. After measuring the wet weight, whole blood samples and ectopic endometrial tissues were digested with supra pure 65% HNO3 and analysed for heavy metals by the Total Reflection X-ray Fluorescence (TXRJF) technique in parts per billion (PPB). Means of the log transformed metal levels were compared using t-tests. RESULTS: Mean (+SD) blood levels of nickel in the cases (2.19±0.45 PPB) was significantly higher than in the controls (0.65±0.14 PPB, p =0.029). The mean (+SD) lead levels in the cases (21.85±1.99 PPB) and controls (17.85 ±1.5 PPB) were similar (p=0.378). Tissue samples had higher mean (+SD) levels of nickel (9.3±15.31 PPB) and lead (66.60±10.37 PPB) as compared to blood levels of nickel (0.502±0.406 PPB) and lead (18.16 ± 1.58 PPB) which were statistically significant (p=0.003 and p=0.025 respectively). CONCLUSIONS: Patients with endometriosis had higher levels of nickel, which is known to exert oestrogenic effects. There is evidence that nickel accumulates in endometrial tissues.