Browsing by Author "Skandaraja, S."

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Antioxident activitiy and α-amylase activity of pressurized water extract of Cinnamomum zeylanicum (L.) bark oil.
(International Research Symposium on Pure and Applied Sciences, 2017 Faculty of Science, University of Kelaniya, Sri Lanka., 2017) Wariyapperuma, W. A. N. M.; Jayawardena, B. M.; Kannangara, S. D. P.; Wijayasinghe, Y. S.; Skandaraja, S.
Cinnamon (Cinnamomum zeylanicum) is an evergreen plant widely grown across Asia as a spice and to extract its essential oil. The composition of essential oil, obtained from different parts of the plant differ in chemical composition. Further, the extraction method would also influence the chemical composition of the essential oil. The aim of the current study was to examine the use of pressurized water to extract oil from Cinnamomum zeylanicum bark and to determine whether this extraction method would give a higher quality oil with enhanced anti-diabetic properties. Bark of Ceylon Cinnamon was powdered and extracted using pressurized water (0.098 MPa). The extract was tested for the total phenolic content (TPC), percentage reducing activity, content of condensed tannin and IC50 values for α-amylase inhibition using Folin-ciocalteu method, vanillin assay and ferric reducing antioxidant power assay respectively. The extract obtained by soxhlet extraction was used as a control. The results showed that percentage yield, the content of total phenolic and content of condensed tannin were 4.24%, 0.065 g gallic acid equivalent/ g extract and 0.012 g catechin equivalent/g extract, while those for the control were 5.16%, 0.19 and 0.015 respectively. The ferric reducing power and the pancreatic α-amylase inhibition (IC50 value) were 203.71 and 50.03 μg/mL for pressurized extract and 260.20 and 38.91 μg/mL for control respectively. Present study reveals that cinnamon bark oil obtained using pressurized water has comparable values for phenolic content, reducing power and α-amylase inhibition compared to soxhlet extraction.
Pressured Water Extraction and Solvent Extraction of Cinnamomum zeylanicum (L.) Bark and Evaluation of Anti-Diabetic Properties.
(In: Proceedings of the International Postgraduate Research Conference 2017 (IPRC – 2017), Faculty of Graduate Studies, University of Kelaniya, Sri Lanka., 2017) Wariyapperuma, W. A. N. M.; Kannangara, S. D. P.; Wijayasinghe, Y. S.; Skandaraja, S.; Jayawardena, B. M.
Pressured water extraction (PWE) is an environmentally friendly technique that has been used to extract anti-diabetic substances than solvent extraction. In the present study, Ceylon Cinnamon(CC, Cinnamomum zeylanicum)was selected as a natural source for anti-diabetic agent. The purpose of this study was to determine the inhibitory potential of α-amylase and αglucosidase by CC bark extracts and to compare the efficiency of the extract with antidiabetic properties from CC by PWE and solvent extraction. The dried bark of CC was powdered and extracted using pressured water (PW, 0.098 MPa), microwave digestion (MD), steam distillation (SD) and soxhlet extraction (SE) with 75% ethanol. The resultant extract from SE was fractionated with hexane. Each extract was tested for the total phenolic content (TPC) and content of proanthocyanidin (PC) using Folin-Ciocalteu method and vanillin assay respectively. IC50 values of α-amylase and α-glucosidase inhibitions by the extracts were determined. Acarbose was used as the positive control. Table 1: TPC, PC and IC50 values of α-amylase and α-glucosidase inhibitions As per the results in table 1 CC water extract obtained by pressured water and aqueous layer from SE have high anti-diabetic properties compared to the other extractions. IC50 values of α-amylase and αglucosidase inhibitions by the acarbose were 95.06±6.84 and 172.52±12.06 respectively. The financial assistance provided by National Science Foundation (SP/CIN/2016/03) is highly appreciated.