Browsing by Author "Panapitiya, P. D. S. N. H."

Filter results by typing the first few letters
Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Antioxidant Capacity and Nutritional Value of Peels and Seeds of Selected Pomegranate (Punica granatum L.) Cultivars from Sri Lanka
    (The Journal of Agricultural Sciences - Sri Lanka, 2022) Panapitiya, P. D. S. N. H.; Weerasooriya, M. K. B.; Punchipatabendhi, T. I.
    Purpose: Nonedible portion of Pomegranate is reported to be rich with a diverse range of phytochemicals which embrace with health promotive features. Though antioxidant power and nutritive value of fruit juice are well known, knowledge of nonedible fraction is very poor. Hence, evaluation of antioxidant power and nutritional value of fruit peel and seed of pomegranates was targeted. Research Method: IC50, Ferric Reducing Antioxidant Power (FRAP), Total Phenolic (TPC), Total Flavonoid (TFC), Total Anthocyanin and pro-anthocyanidin Contents (ProAC) were determined. Nutritional value was studied by proximate analysis. Findings: IC50, TPC, FRAP and TFC values of peels were ranged from 4.6 to 41.1μg/mL, 318±1.77 to 478±5.62 mg Gallic acid equivalent/g, 4.270±0.83 to 6.690±0.15 mM Fe2+/g and 52.64±0.24 to 75.99±0.849 Rutin equivalent mg/g respectively. Antioxidant power and TFC of all the peel extracts were well above as compared with juice and seed samples, the highest IC50 and TPC in Daya peel whereas the highest FRAP and TFC in Nimali and Kalpitiya red peels respectively. Kalpitiya red juice and peel had the highest TAC and ProAc. Proximate analysis revealed that Protein, lipid and fiber contents were higher in seeds than peels. Carbohydrate content of all the peels was higher than the seeds. Research Limitations: There were some practical limitations such as long dry spells and also finding suitable fields for the experiment, due to farmers’ hesitation on possible yield reduction. Originality/ Value: Findings reveal that selected cultivars of pomegranate peel possess exceptionally high antioxidant power and could be applied as an excellent source of natural antioxidant in future therapeutic and medicine and as a safer natural antioxidant in food industries. High nutrient contents in pomegranate by-products facilitate to develop nutritionally valuable components such as functional food ingredients and nutraceuticals.
  • Thumbnail Image
    Item
    Evaluation and comparison of antioxidant properties and total phenolic content of pomegranate (Punica granatum L.) peel and juice
    (4th International Research Symposium on Pure and Applied Sciences, Faculty of Science, University of Kelaniya, Sri Lanka, 2019) Panapitiya, P. D. S. N. H.; Weerasooriya, M.K.B.
    Pomegranate peel is currently a waste but contains valuable, rich with a diverse range of bioactive compounds. The objective of the present study was to assess the antioxidant properties by IC50 value and FRAP value, total phenolic content (TPC), total flavonoid content (TFC) and total anthocyanin content (TAC) of the peel (PP) and the juice (PJ) of local pomegranate cultivars, Nayana, Nimali and Kalpitya red. Antioxidant properties were tested by FRAP and DPPH assay. TPC was measured spectrophotometrically using the Folin Ciocalteu method and expressed as mg Gallic acid equivalents (GAE)/gram. TFC was evaluated by the aluminum chloride assay. TAC was detected by the vanillin assay. The TAC and TFC of PP of Nimali, Nayana and Kalpitya red cultivars were 22.12 ± 0.20, 49.05 ± 0.30 and 69.08 ± 0.57 mg catechin equivalents (CE)/g and 67.67 ± 0.59, 59.58 ± 0.85 and 75.99 ± 0.85 mg rutin equivalent (RE)/g respectively. The TAC of PJ ranged from 0.40 ± 0.01 to 2.82 ± 0.03 mg CE/g was nearly 60 times less than that of PP of the same cultivar. The TFC of PJ ranged from 0.39 ± 0.00 to 0.80 ± 0.00 mg RE/g was nearly 100 times less than that of PP. The TPC and FRAP values of PP of Nimali, Nayana and Kalpitya red cultivars were 469.67 ± 8.97, 413.92 ± 4.75 and 318.82 ± 4.66 mg GAE/g and 6690.00 ± 153.00, 4270.60 ± 83.00 and 4512.00 ± 14.00 μmol Fe2+/g respectively. The TPC of PJ varied from 0.87 ± 0.02 to 1.98 ± 0.04 mg GAE/g was nearly 300 times less than that of PP. The FRAP values of PJ ranged from 15.57 ± 0.36 to 40.18 ± 0.29 μmol Fe2+/g were nearly 200 times less than that of PP. The IC50 values of PP of Nimali, Nayana and Kalpitya red cultivars were found to be 14.20, 20.40 and 18.00 μg/mL respectively. The IC50 values of PJ were in the range of 915-3098 μg/mL, nearly 100 times higher than that of PP. The results revealed that PP contains exceptionally high TPC and antioxidant properties observed by IC50 and FRAP values with respect to PJ of the same cultivar. The PP of Nimali cultivar had the highest antioxidant properties and TPC. The PP of Kalpitiya red cultivar possessed the highest TFC and TAC. Thus, PP has a high potential to be utilized as a resource of bioactive compounds. The results proved that there is no correlation between phenolic compounds and the antioxidant properties. All the parameters tested were significantly different (p<0.05) among the cultivars.