Browsing by Author "Nageeb, B.M."

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A Comparative Preliminary Study on the Prevalence of Overweight-Obese with Socio-Economic Status (SES) Among the Adult Females
(1st International Conference on Unani, Ayurveda, Siddha and Traditional Medicine, On Natural Solutions for Health Challenges, 2013) Manuha, M.I.; Iqbal, N.Z.; Nageeb, B.M.; Paranagama, P.A.
This study was to determine how the SES influenced on overweight and obesity in adult women. The analysis was done by ISBM SPSS. BMIwas categorized according to WHO criteria such as normal weight: 18.5-24.9 kg/m2; overweight: 25.0- 29.9 kg/m2; or obese > 30.0 kg/m2. Obese further categorized to obese-I (30-34.9 kg/m2), obese-II (35-39.9 kg/m2) and morbid obese ( > 40 kg/m2). 206 participants were participated. In this study 32.5% were overweight and 67.5% were obese. Out of this 67% of obese 43.2% were belong to obese I, 17.5% were belong to obese II and 6.8% were in the morbidly obese. 43.3%, 40.3% of overweight were found in the age group 31-40 and 41-60 respectively. Similarly 35.7%, 47.4% of obese were found in the similar age groups. 53.7% was overweight and 68.3% was obese found in the participants who completed or below level of primary education. Further in the participants who completed the secondary education or above, the percentage of overweight (31.4%) was found lesser amount than obesity (46.3%). Family income concerned both 33.3% overweight and 37.4% obese were found in less income family (LKR < 25000). 29.8% overweight and 31.7% obese were found in the families who received the income between LKR 25000 - 50000. Inthis study 71.8%, 22.3 %, 5.8% were house wives, employees and students respectively. Overweight/obese found more in the age groups between 31-60. Overweight/obese found higher percentage in poor educated group. This study indicates that with the increasing of the age the overweight condition is transforming into obese condition. Therefore a study on wide range of the population is needed to come to a conclusion.
A preliminary study of anti-bacterial effect of selected five Ayurvedic compound preparations.
(2006) Nageeb, B.M.; Widanapathirana, S.; Amarasinghe, A.P.G.
Plant based medicaments have been man?s prime therapeutic weapons to rescue him from disease. Plants are of relevance to pharmacology. Pharmacological properties of medicinal plants may be used as leads in developing modern therapeutic agents. Thalisadee, Thripala,Hinguastaka, Dathree, and Manibadra are common Ayurvedic formulae used in traditional system of medicine in Sri Lanka. Thalisadee and Thripala Choorna are being commonly used in respiratory disorders such as cough, cold bronchitis and fever. The Hinguastaka, Dathree and Manibadra Choorna are being commonly used in gastrointestinal disorders such as diarrhoea dysentery vomiting and indigestion. Most of these conditions may develop due to bacterial infections .The main objective of this study was to evaluate the antibacterial effect of these preparations. Minimum human single dose of these drugs (2.5 gram) was dissolved in sterile distilled water and kept in the shaker at 100 rpm continuously for 04 hours in order to get the maximum soluble liquid extract of these drugs. 0.7 gram of Nutrient broth was dissolved in 50 ml of distilled water and transferred in to five test tubes (10 ml. /tube) and sterilized by autoclaving at 121 C for 20 minuets. These Nutrient broth tubes were inoculated by using inoculating needle with already plated pure test cultures of Pseudomonas aeruginosa, ,Escherichia coli , Salmonella typhi.These tubes were incubated at 37 0 C for 18 to 20 hours. Sterilized Nutrient agar was transferred into ten sterilized Petri dishes at 40 0C and allowed to solidify on a horizontal plane. These plates were sealed and kept in incubator at 37 0C for 24 hours to exclude any contaminations and to reduce the moisture content. A known amount of (0.05 ml) each culture broth containing specific organisms was added to these solidified agar plates and spread evenly using a sterilized glass spreader. On these seeded agar plates sterilized metal cylinders were kept (03 Cylinders/plate) with gentle pressure. These cylinders were filled with 0.1 ml of above liquid extract of drug preparations.De ionized sterilized distilled water 0.1 ml and Chlorampenicol 0.025 mg/0.1 ml were used as controls. These plates were sealed and incubated at 37 0C .This same procedure was repeated for three times for each of the test organism. Chlorampenicol showed 1.0 cm -1.5 cm clear inhibition zones of the bacterial lawns on every test organisms. None of the drug preparations showed any effect on Escherichia coli culture plates. The Ayurvedic compound preparations of Hinguastaka Choorna and Manibadra Choorna extracts showed averagely 0.5cm and 0.3 cm clear inhibition zones of the bacterial lawns on Salmonella typhi plate respectively. Thripala Choorna showed averagely 1.0 cm clear inhibition zone of bacterial lawns on Pseudomonas aeruginosa plates. These zones were clear on every repetition. Theses results were statistically analyzed by using one sample student T-Test. All the means are in between accepted level and P value is <0.05. Comparison to the Chlorampenicol,Higuastka Choorna and Manibadra Choorna are active against Salmonella typhi. Thripala Choorna is active against Pseudomonas aeruginosa. Both, Dathree Choorna and Thalisadee Choorna are not active against any of tested microorganisms. This preliminary study scientifically justifies that the use of the powder preparations of Higuastka Choorna,Manibadra Choorna and Thripala Choorna for infective conditions such as diarrhea, dysentery, vomiting and indigestion.
A Preliminary Study on Microbial Quality Standards of an Ayurvedic Compound Preparation "Thalisadee Choorana".
(2007) Nageeb, B.M.; Widanapathirana, S.; Amarasinghe, A.P.G.; Kasthuriarchi, K.A.H.
Thalisadee choorana is a common Ayurvedic medical preparation widely used by all indigenous medical practitioners in Sri Lanka. It is used for respiratory tract ailments such as cough, common cold, bronchitis, asthmatic conditions and gastro intestinal disorders such as diarrhoea, vomiting, indigestion and loss of appetite. It contains mainly Pipemigram (Gammiris),Piperlongum(Thippili), Abies�wehhiuna (Thalispathra),Cinnamum zylanicum (Kurundupothu), Elettaria repens( Heenensal),Bamboo salt (Unakapuru) and sugar. All plant materials contain a large number of microorganisms.Some are inherent and some are contaminated during the process of harvesting and manufacturing process. Considering these facts, the World Health Assembly in its resolutions WHA-31 :33(1978) 40:33(1987), 42:43(1989) has emphasized the need of ensuring the quality in regard to microbial content of the plant products. Hence this study was carried out to determine the microbial load of this product and the possible sterilization methods of reducing the microbial load. The effect of the method on the drug which reduces the microbial load of the drug also studied. Ten different samples of Thalisadee choorana were subjected to this study. 0.1 gram of the drug sample was dissolved in 10 ml of sterile distilled water. (10�). Using this solution 10-1, 10-2 10-3 dilutions were prepared. Routine sterilization procedures were carried out in all steps.Nutrient agar and Potato dextrose agar were used as general culture media. Pour plate technique and spread plate technique were used to detect the microbial count respectively. 0.1 ml of above dilutions was used on culture plates. Each plate was controlled by using another duplicate culture plate. Plates were sealed and kept under normal room temperature. Colony counts were taken after 24 hours and 72 hours for bacteria and fungi respectively. It was assumed that each colony was formed by a single organism. Same procedure was repeated three times. According to the W.I-I.A standard, aerobic bacteria up to 105 I gram, yeast and moulds up to 103 I gram arc permitted The results ofthe above study indicate that the bacterial count was in between 3x106 to 4xl06 /gram. These results indicate that the limits were exceeding on every sample. The following methods were tried to reduce the microbial load. I 00 grams of the above samples were subjected to (a) Heat treatment in a hot air oven at 80� C for 10 minuets for three consecutive days. (b) Ultra violet radiation at 256 wave length continuously for 24 hours. (c) Steam treatment under atmospheric pressure in a closed container for 10 minuets for three consecutive days. The study of microbial load was thereafter repeated.The plates of the steamed samples were sterile up to 72 hours while the plate of other two methods does not show any reduction in microbial load. The volatile oil content by reflux method using Dead and Stark apparatus and the thin layer chromatographic (T.L.C) patterns of Ethanol extract and Water extracts using Silica gel GF 254 and G06 at the ratio of 1:3 with several solvent systems of both samples (Steamed and un steamed) were studied. The T.L.C. patterns and the volatile oil content of both samples were comparatively same .This preliminary study reveals that the steam treatment method is comparatively an effective method to reduce the microbial load of the above preparation. A detail study of the chemical compounds through other chromatography methods is needed to confirm this.
A study on selected biometrics and central obesity indices among
(Book of Abstracts, Annual Research Symposium 2014, 2014) Manuha, M.I.; Nageeb, B.M.; Iqbal, N.Z.; Paranagama, P.A.
The study was to analyze the relationship between body mass index (BMI) of overweight/obesity adult women with central obesity indices such as waist circumference (WC), Hip circumference (HC), waist-to-hip ratio (WHR) with lipid profile such as total cholesterol (TC), high-density lipoprotein (HDL), triglyceride (TG) and low-density lipoprotein (LDL). Percentage of overall abnormal lipid levels and low haemoglobin level in overweight and obese were also analyzed. Female adults with overweight (BMI between 25 -30 Kg/m2 or obese (BMI over 30 Kg/m2) and the ages ranged between 18- 60 years were included in this study. The subject studied constituted a convenience sample of 121 representing different socio-economic status. Measurements; Body weight, height, waist and hip circumferences, blood pressure, pulse, lipid profile with TC, HDL, TG, LDL and Hb percentage were investigated. The results showed that 63.6% with high LDL-C, 59.1 % with high TC, 18.2 % with high TG and 43.2% with low HDL-C. Haemoglobin levels of the participants were 47.9% and it is below the range of cut off value given by WHO. Correlation between BMI and WC, HC, WHR was analyzed separately. According to the results obtained BMI showed a strong positive correlation with WC and HC, n (121) =0.8, p < 0.01 and n(121) = 0.838, p < 0.01 respectively. Analysis of correlation between WC with HC and WHR indicated strong positive correlation, n (121) =0.809, p < 0.01 and n (121) = 0.442 respectively. TC and LDL were strongly positively correlated, n (121) =0.895 p < 0.01, but the HDL was inversely associated with TG and LDL P = -0.072, P = -0.184 respectively. TG was inversely associated with LDL-C (P= - 0.072).Concurrently, the abnormally highest mean of WC 105.4 � 11.7cm and the highest mean of waist to hip ratio 0.97 �0.05 also found. In the present study the results revealed that more than half of overweight and obese adult women had abnormal lipid levels and nearly half of overweight and obese adult women had low hemoglobin concentration. The present study revealed a higher percentage of body fat across a range of waist circumference values. The mean waist circumference, hip circumference and waist to hip ratio were significantly higher than the cut off points reported by WHO (2008).
A study on the correlation between Body Mass Index of overweight/obese, Total PhysicalActivity and Cardiovascular risk factors with special reference to dyslipidaemia in adult women
(Sri Lanka Journal of Indegenous Medicine (SLJIM), 2013) Manuha, M.I.; Iqbal, N.Z.; Nageeb, B.M.; Paranagama, P.A.
MI1,NZ 2, BM1, P Paranagama3
Association of Physical Activity and Sedentary Lifestyle with Overweight and Obesity among Adult Women in Sri Lanka
(2Department of Chemistry, University of Kelaniya, Kelaniya, Sri Lanka, 2013) Manuha, M.I.; Nageeb, B.M.; Iqbal, N.Z.; Paranagama, P.A.
A comparative preliminary study of anti-bacterial effect of ayurvedic compound preparations of Dathree choorna and Hinguastaka choorna
(Sri Lanka Association for the Advancement of Science, 2006) Nageeb, B.M.; Amarasinghe, A.P.G.; Widanapathirana, S.
Determination of Vitamin C (Ascorbic acid) in lime and lemon
(Institute of Indigenous Medicine, University of Colombo, 2014) Manuha, M.I.; Iqbal, N.Z.; Nageeb, B.M.; Paranagama, P.A.
Evidence based Unani basic factors that would effective on the management of obesity in this new era
(Book of Abstracts, Annual Research Symposium 2014, 2014) Manuha, M.I.; Nageeb, B.M.; Iqbal, N.Z.; Paranagama, P.A.
Improper diet habits and lethargy for exercises are mentioned widely for the causes of obesity1. Though the causes were found and many programs have been conducted, the obesity is still a challenging problem all over the world. Therefore, the objectives of this study were to identify causes of obesity in ancient Unani concept through the modern interpretation. The ancient physician Avicenna mentioned in his book ?Canon of Medicine� that functional disorder in the Natural faculty (Quwat e Tabai�yya ) is one of the main causes for the obesity with the above said causes2. According to the Unani or Greek concept the system called ?Natural faculty� which governs digestion, metabolism, nutrition and growth of the body. A functional disorder in the natural faculty may occur due to weakness in the organs of natural faculty or due to emotional disturbances such as anger, sadness, jealousy etc. 2. A systematic search was carried out using many ancient texts including ?Avicenna�s Canon on Medicine�, Unani Materia Medica text and scientific research journal articles located in the PUBMED journals. The review was aimed to collected published data on utilization of herbs to reduce obesity. Articles in 61 journals were cited in the systematic reviews and 58.3% research based on animal studies, 33.3% clinical research found on humans and another 8.3% was on the literary review of the anti-obesity herbs. These researches exhibit that there was a significant reduction of weight found after the herbal treatment on the obese mice or obese human. From this review it was found that 95.8% herbs possess the action of strengthening the Natural faculty or gastro intestinal tract (GIT). Out of this 54.2% of herb or part of a herb used in this research had the action of carminative effect on GIT and 45.8% herb or herb part used here had the actions of carminative and strengthening effect on GIT. Hence it was concluded that according to Unani concept a special attention should be focused to enhance the right functions of the natural faculty by carminatives medicines such as cumin etc. when treating obesity by regulating diet and exercise.
Haemoglobin level among overweight and obese adult women
(National Centre for Advanced Studies, 2014) Manuha, M.I.; Iqbal, N.Z.; Nageeb, B.M.; Paranagama, P.A.
Human Red Blood cell (HRBC) membrane stabilization as a mechanism of the anti-inflammatory activity of aqueous and methanol extract of a compound preparation which used to reduce weight on obese patients
(Institute of Indigenous Medicine, University of Colombo, 2014) Manuha, M.I.; Iqbal, N.Z.; Nageeb, B.M.; Paranagama, P.A.
Impact of a native ‘natural spice mixture’ on selected biomarkers, reduction of excess weight in purposely selected overweight/obese females
(Faculty of Graduate Studies, University of Kelaniya, 2015) Manuha, M.I.; Iqbal, N.Z.; Nageeb, B.M.; Paranagama, P.A.
The objective of this study is to explore the changes in the body weight and selected biomarkers of overweight/obese women after a four month intervention of native ‗natural spice mixture‘. This study was done at National Ayurveda Teaching Hospital, Borella. Volunteers (129) were participated, and BMI and selected biomarkers (lipid profile) were measured before and after intervention. Chi square test revealed that there is a significant weight reduction after the intervention (Chi Square = 36.74, df = 9, p< 0.05). Although there was no participant with a normal BMI before intervention, 7 % of participants have attained normal BMIs after the intervention. The BMI range 25-29.9 (overweight) have increased by decreasing the ranges 30 – 34.9 (Obesity-1), 35 - 39.9 (Obesity-2) and >=40 (Morbid obesity). Before intervention the percentages of ‗High‘ and ‗Borderline high‘ cholesterol were 17.8 % and 38.8 %, respectively and after the intervention the same were reduced to 7 % and 22.7 %. Before intervention only 43.4 % of the participants were with the normal total cholesterol range, but it increased to 70.3 % after intervention with a significant (p < 0.05) change. Only 6.2 % belonged to the ‗High triglyceride‘ category before the intervention and after the intervention it reduced to 2.2 %. Percentage of ‗Optimal‘ and ‗Near optimal‘ levels of LDLs increased from 17.1 % to 27.6 % and from 28.7 % to 39.4 % after the intervention, respectively. The ‗Borderline‘ and ‗High‘ levels of LDLs were reduced by 5.8 % and 11.6 % after the intervention, respectively. Study shows that 19.4 % of the participants had ‗low HDL‘ before the intervention and it increased to 23.4 % after the intervention. The intervention of ‗natural spice mixture‘ revealed the promising effect on weight reduction and rearranged the dyslipidaemia.
A microbiological study of an Ayurvedic compound preparation Dasamoola Arista with a view to defining an acceptable microbial quality standard
(Sri Lanka Association for the Advancement of Science, 2008) Nageeb, B.M.; Widanapathirana, S.; Amarasinghe, A.P.G.
The Indigenous system of medicine has been practiced successfully over several thousand years. The basic ingredients of indigenous medicine are plant materials. These materials contain natural inherent microbial flora and also may become contaminate during processing. Considering these facts the World Health Assembly in its resolutions WHA-31:33, 40:33, and 42:43 has emphasized the need for the microbial quality standard of medicinal plant products. Dasamoola Arista, has been used in therapeutics for several centuries. The objectives of this study were to enumerate the total viable count of bacteria, fungi and specific microorganisms such as Coliforms and Salmonella in the market samples of this drug. Fourteen different market samples were subjected to this study. Nutrient agar and Potato Dextrose agar were used as culture media. Pour plate and Spread plate techniques were used to study the microbial load in dilution series up to 10-3. Microbial counts on Nutrient agar and Potato dextrose agar were taken after 24 hours and 72 hours. Tests for Coliforms and Salmonella were done according to International standards. Coliform test was performed by MPN method using single strength MacConkey broth. Salmonella was tested after an enrichment process in buffered peptone. 0.1ml of this peptone was transferred to test tubes of Tetrathionate and Selenite broth separately and Incubated at 37 0C for 48 hours. These broths were streaked on Bismuthsulphiteagar (B/S.Agar) and Brilliantgreenbile agar(BGB ) Black colonies on B/S-Agar and Pink colonies on BGB Agar were considered as positive for Salmonella. These colonies were bio chemically tested for salmonella . All tests were repeated thrice and results were confirmed. The microbial load observed in this study was with in the limits of the WHA. The Colony count for Bacteria was in between 10x10 to 10x68. Fungi Colony count was in between 1x10 to 36x 10. The biochemical tests revealed that the Bacteria present in this preparation was Bacillus firmus. None of the drug samples were positive for Coliforms or Salmonella. These results revealed that these tested samples were microbiologically safe and up to the microbial quality standard.
A preliminary study of anti-bacterial effect of selected five Ayurvedic compound preparations
(University of Kelaniya, 2006) Nageeb, B.M.; Widanapathirana, S.; Amarasinghe, A.P.G.
Plant based medicaments have been man’s prime therapeutic weapons to rescue him from disease. Plants are of relevance to pharmacology. Pharmacological properties of medicinal plants may be used as leads in developing modern therapeutic agents. . Thalisadee, Thripala, Hinguastaka, Dathree, and Manibadra are common Ayurvedic formulae used in traditional system of medicine in Sri Lanka. Thalisadee and Thripala Choorna are being commonly used in respiratory disorders such as cough, cold bronchitis and fever. The Hinguastaka, Dathree and Manibadra Choorna are being commonly used in gastrointestinal disorders such as diarrhoea dysentery vomiting and indigestion.. Most of these conditions may develop due to bacterial infections .The main objective of this study was to evaluate the antibacterial effect of these preparations. Minimum human single dose of these drugs (2.5 gram) was dissolved in sterile distilled water and kept in the shaker at 100 rpm continuously for 04 hours in order to get the maximum soluble liquid extract of these drugs. 0.7 gram of Nutrient broth was dissolved in 50 ml of distilled water and transferred in to five test tubes (10 ml. /tube) and sterilized by autoclaving at 1210C for 20 minuets. These Nutrient broth tubes were inoculated by using inoculating needle with already plated pure test cultures of Pseudomonas aeruginosa, ,Escherichia coli , Salmonella typhi, .These tubes were incubated at 37 0 C for 18 to 20 hours. Sterilized Nutrient agar was transferred into ten sterilized Petri dishes at 40 0 C and allowed to solidify on a horizontal plane. These plates were sealed and kept in incubator at 37.0C for 24 hours to exclude any contaminations and to reduce the moisture content. A known amount of (0.05 ml) each culture broth containing specific organisms was added to these solidified agar plates and spread evenly using a sterilized glass spreader. On these seeded agar plates sterilized metal cylinders were kept (03 Cylinders/plate) with gentle pressure. These cylinders were filled with 0.1 ml of above liquid extract of drug preparations. De ionized sterilized distilled water 0.1 ml and Chlorampenicol 0.025 mg/0.1 ml were used as controls. These plates were sealed and incubated at 370C .This same procedure was repeated for three times for each of the test organism. Chlorampenicol showed 1.0 cm -1.5 cm clear inhibition zones of the bacterial lawns on every test organisms. None of the drug preparations showed any effect on Escherichia coli culture plates. The Ayurvedic compound preparations of Hinguastaka Choorna and Manibadra Choorna extracts showed averagely 0.5 cm and 0.3 cm clear inhibition zones of the bacterial lawns on Salmonella typhi plate respectively. Thripala Choorna showed averagely 1.0 cm clear inhibition zone of bacterial lawns on Pseudomonas aeruginosa plates. These zones were clear on every repetition. Theses results were statistically analyzed by using one sample student T-Test. All the means are in between accepted level and P value is <0.05. Comparison to the Chlorampenicol, Higuastka Choorna and Manibadra Choorna are active against Salmonella typhi. Thripala Choorna is active against Pseudomonas aeruginosa. Both, Dathree Choorna and Thalisadee Choorna are not active against any of tested microorganisms. This preliminary study scientifically justifies that the use of the powder preparations of Higuastka Choorna, Manibadra Choorna and Thripala Choorna for infective conditions such as diarrhea, dysentery, vomiting and indigestion.
A Preliminary Study on Microbial Quality Standards of an Ayurvedic Compound Preparation "Thalisadee Choorana"
(University of Kelaniya, 2007) Nageeb, B.M.; Widanapathirana, S.; Amarasinghe, A.P.G.; Kasthuriarchi, K.A.H.
Thalisadee choorana is a common Ayurvedic medical preparation widely used by all indigenous medical practitioners in Sri Lanka. It is used for respiratory tract ailments such as cough, common cold, bronchitis, asthmatic conditions and gastro intestinal disorders such as diarrhoea, vomiting, indigestion and loss of appetite. It contains mainly Pipemigram (Gammiris), Piperlongum(Thippili), Abies·wehhiuna (Thalispathra), Cinnamum zylanicum (Kurundupothu), Elettaria repens( Heenensal),Bamboo salt (Unakapuru) and sugar. All plant materials contain a large number of microorganisms. Some are inherent and some are contaminated during the process of harvesting and manufacturing process. Considering these facts, the World Health Assembly in its resolutions WHA-31 :33(1978) 40:33(1987), 42:43(1989) has emphasized the need of ensuring the quality in regard to microbial content of the plant products. Hence this study was carried out to determine the microbial load of this product and the possible sterilization methods of reducing the microbial load. The effect of the method on the drug which reduces the microbial load of the drug also studied. Ten different samples of Thalisadee choorana were subjected to this study. 0.1 gram of the drug sample was dissolved in 10 ml of sterile distilled water. (10°). Using this solution 10- 1, 10-2 10-3 dilutions were prepared. Routine sterilization procedures were carried out in all steps. Nutrient agar and Potato dextrose agar were used as general culture media. Pour plate technique and spread plate technique were used to detect the microbial count respectively. 0.1 ml of above dilutions was used on culture plates. Each plate was controlled by using another duplicate culture plate. Plates were sealed and kept under normal room temperature. Colony counts were taken after 24 hours and 72 hours for bacteria and fungi respectively. It was assumed that each colony was formed by a single organism. Same procedure was repeated three times. According to the W.I-I.A standard, aerobic bacteria up to 105 I gram, yeast and moulds up to 103 I gram arc permitted The results ofthe above study indicate that the bacterial count was in between 3x106 to 4xl06 /gram. These results indicate that the limits were exceeding on every sample. The following methods were tried to reduce the microbial load. I 00 grams of the above samples were subjected to (a) Heat treatment in a hot air oven at 80° C for 10 minuets for three consecutive days. (b) Ultra violet radiation at 256 wave length continuously for 24 hours. (c) Steam treatment under atmospheric pressure in a closed container for 10 minuets for three consecutive days. The study of microbial load was thereafter repeated. The plates of the steamed samples were sterile up to 72 hours while the plate of other two methods does not show any reduction in microbial load. The volatile oil content by reflux method using Dead and Stark apparatus and the thin layer chromatographic (T.L.C) patterns of Ethanol extract and Water extracts using Silica gel GF 254 and G06 at the ratio of 1:3 with several solvent systems of both samples (Steamed and un steamed) were studied. The T.L.C. patterns and the volatile oil content of both samples were comparatively same .This preliminary study reveals that the steam treatment method is comparatively an effective method to reduce the microbial load of the above preparation. A detail study of the chemical compounds through other chromatography methods is needed to confirm this.
Prevalence of obesity related health risk conditions among overweight and obesity adult women
(1st International Conference on Unani, Ayurveda, Siddha and Traditional Medicine, On Natural Solutions for Health Challenges, 2013) Manuha, M.I.; Iqbal, N.Z.; Nageeb, B.M.; Paranagama, P.A.
A study of total viable count of microorganism and specific microorganisms in four selected Arista and Asawa preparations
(Sri Lanka Association for the Advancement of Science, 2009) Nageeb, B.M.; Amarasinghe, A.P.G.; Widanapathirana, S.
A study on selected biometrics and cetral obesity indices among overweight and obese adult women
(University of Kelaniya, 2014) Manuha, M.I.; Iqbal, N.Z.; Nageeb, B.M.; Paranagama, P.A.