Browsing by Author "Mitui, M.T."

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    Inaccurate identification of rotavirus genotype G9 as genotype G3 strains due to primer mismatch
    (BioMed Central, 2012) Mitui, M.T.; Chandrasena, T.G.A.N.; Chan, P.K.S.; Rajindrajith, S.; Nelson, E.A.S.; Leung, T.F.; Nishizono, A.; Ahmed, K.
    Reverse transcription (RT)-PCR is now the standard method for typing group A rotaviruses (RVA) to monitor the circulating genotypes in a population. Selection of primers that can accurately type the circulating genotypes is crucial in the context of vaccine introduction and correctly interpreting the impact of vaccination on strain distribution. To our knowledge this study is the first report from Asia of misidentification of genotype G9 as G3 due to a primer-template mismatch. We tested two published G-genotype specific primers sets, designed by Gouvea and colleagues (Set A) and Iturriza-Gomara and colleagues (Set B) on RVA from Hong Kong and Sri Lanka. Among 52 rotaviruses typed as G3 by set A primers, 36 (69.2%) were identified as G9 by nucleotide sequencing and set B primers. Moreover, of 300 rotaviruses tested, 28.3% were untypable by set A primers whereas only 12.3% were untypable by set B primers. Our findings reinforce the need to periodically monitor the primers used for RVA genotyping.
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    Rotavirus infections with multiple emerging genotypes in Sri Lanka
    (Springer-Verlag, 2010) Ahmed, K.; Batuwanthudawe, R.; Chandrasena, T.G.A.N.; Mitui, M.T.; Rajindrajith, S.; Galagoda, G.; Pun, S.B.; Uchida, R.; Kunii, O.; Moji, K.; Abeysinghe, N.; Nishizono, A.; Nakagomi, O.
    Rotavirus diarrhea is an important cause of child mortality in developing countries, but studies on this diarrhea are scarce in Sri Lanka. A prospective study conducted in Sri Lanka on rotavirus infection among children in a hospital setting (n = 611) versus children residing in tsunami camps (n = 52) showed that prevalence of rotavirus infection was comparable, 21.9 and 20%, respectively. The hospital and camps were located in different districts. Analysis of the genotypes of 122 rotaviruses from the hospital and 12 from the camps indicated that G9P[8] was associated with 35 and 33%; G12P[8/nt] with 14.7 and 33%; G3P[8/4/nt] with 17 and 8% and G1P[8/4] with 6.5 and 16.7%. Rotaviruses with G2P[8/4/6] and G4P[8/4] were hospital-associated only, and some rotaviruses (9 and 8% from the hospital and the camps, respectively) were G- and P-nontypable. We conclude from the present study that multiple emerging genotypes were prevalent in Sri Lanka, and children in camps were at risk of developing diarrhea due to rotaviruses