Browsing by Author "Karunanayake, K. O. L. C."
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Item Pathogenicity of Stem-end Rot Associated Fungi Isolated from Karthakolomban Mango and Their Control by Spray and Fumigation Treatments with Selected Essential OilsPathogenicity of Stem-end Rot Associated Fungi Isolated from Karthakolomban Mango and Their Control by Spray and Fumigation Treatments with Selected Essential Oils(The Journal of Agricultural Sciences - Sri Lanka, 2020) Kodituwakku, T. D.; Abeywickrama, K.; Karunanayake, K. O. L. C.Purpose: Stem-end rot (SER) is a major disease of mango that causes serious postharvest losses. Application of fungicides is environmentally unsound and is being practiced currently in its control. This study was conducted to develop essential oil treatment systems as eco-friendly strategies to control SER of Karthakolomban mango and to determine the pathogenicity of several SER associated fungi. Research Method: Pathogenicity of four fungal isolates in SER was assessed by inoculating them on Karthakolomban mango fruits. Karthakolomban mango fruits were subjected to spray and fumigation treatments using four essential oils and their pathological, physicochemical and sensory properties were evaluated after a 8-day storage at 12 – 14 ºC. Findings: Lasiodiplodia theobromae, Pestalotiopsis sp., Phomopsis sp. and Xylaria feejeensis were the major SER pathogens of mango. X. feejeensis was identifi ed as a SER pathogen for the fi rst time in Sri Lanka. Spray and fumigation treatments conducted using basil, clove, cinnamon leaf and cinnamon bark oils eff ectively controlled SER of Karthakolomban mango stored at 12 – 14 ºC for 8 days. Mango sprayed with 1.6 μL/mL cinnamon bark oil has displayed no SER after induced ripening. None of the treatments caused drastic alterations in physicochemical and sensory properties of mango. Research Limitations: Storage period of mango was restricted to 8 days due to the initiation of natural ripening which was the major limitation to achieving a further enhanced shelf life. Originality/value: The treatment strategies developed by this research could be commercialized as biosafe SER control strategies in reducing postharvest losses of mango in the local and international trade.Item The spray of essential oils with passive modified atmosphere packaging to control stem-end rot of mango 'Karutha Golomban'(lnternational Society for Horticultural Science, lll Asian Horticuttural Gongress 'AHC2020, 2020) Kodituwakku, T. D.; Abeywickrama, K.,; Karunanayake, K. O. L. C.'Karutha Cotomban' mango is more prone to stem-end rot (SER) disease that causes substantial postharvest losses in quality as well as quantity. The objective of this study was to use aqueous emulsions of basil (1.6 pL/mL), clove (2.0 pL/mL), cinnamon leaf (2.0 pL/mL) and cinnamon bark (1.6 pL/mL) oils as sprays with passive modified atmosphere packaging (MAP) in low density polyethylene (LDPE) bags qs_ a bio-s_afe strategy to control SER of 'Karutha Colomban' mango stored at 12 - 14 "C and 85 - g0% of retative humidity. Distilled water and 0.1% (w/v) carbendazim treatments were used as negative and positive controls respectively. ln-package respiratory gases were measured during storage for B days. Pathological, physicochemical, sensory evaluation and feel color of mango were assessed after transferring to ripening at the end of the storage period. All essential oil treatments combined with passive MAP have significantly reduced SER severity of mango to 0.3 ' 1.1% when compared to the negative controlwithout adversely affecting the physicochemical, sensory properties anl peel color. Mango fruits treated with basil, clove and cinnamon leaf oils obtained a slighily higher preference by the sensory panelthan other treatments. ln-package 02 concentration was at 3.8 - 5.8%, while CO2 was at 4.3 - 5.0% at the end of the B-day storage period. Respiratory gas levels attained a steady state equilibrium which resulied'in extending the shelf life by reducing the metabolism of both commodity and associated microorganisrns. Further, current treatments can be commercialized as eco-friendly treatment strategies to be used during transportation and storage of mango in local trade within one week and for exportation via air cargo that takes approximately 2 - 3 daYs.