Browsing by Author "Jayawardane, B. M."

Now showing 1 - 2 of 2

Determination of anti-diabetic properties in Cinnamomum zeylanicum
(Research Symposium on Pure and Applied Sciences, 2018 Faculty of Science, University of Kelaniya, Sri Lanka, 2018) De Silva, W. D. T.; Jayawardane, B. M.
Diabetes is one of the major complications that millions of individuals in the world faces today. If two key enzymes, the α amylase and α glucosidase are inhibited, it can lower the possibility to develop diabetes. Synthetic anti-diabetic drugs are associated with various gastrointestinal side effects. Therefore, it is important to identify and explore natural sources that have fewer side effects for managing diabetes. The aim of this study was to evaluate anti diabetic properties in Cinnamomum zeylanicum leaf . The C . zeylanicum leaves were collected from a cinnamon cultivation at Waskaduwa area and extracted by pressurized hot water extraction method and Soxhlet extraction method with ethanol . Each extract was tested for flavonoid content, phenolic content, radical scavenging effect and inhibitory potential against α amylase and α glucosidase enzymes . Flavonoid content was determined by the aluminium chloride colorimetric method and radical scavenging activity was determined using DPPH assay in triplicates . α amylase inhibitory activity and α glucosidase inhibitory activity were measured in duplicates by starch iodine method and enzyme inhibition method based on para nitrophenyl α D glucopyranoside (pNPG) substrate respectively . The highest content of flavonoids (52 . 23 ± 3 . 87 µg Quarcetin equivalent /100 g) was present in ethanolic extract . Phenolic content was determined by Folin Ciocalteu colorimetric method . The highest content of phenolics (110 . 57 ± 1 . 71 µg Gallic acid equivalent/g) was observed in water extract . At 1 mg/mL, the highest radical scavenging effect was observed in ethanolic extract (66.98% ± 2.93) relative to water extract. Both extracts showed inhibitory potential against α - amylase (IC50 of ethanolic extract 0 . 0956 mg / mL, water extract 0 . 4880 mg/mL, Acarbose 0 . 0536 mg/mL) and α - glucosidase (IC50 of ethanolic extract 0 . 1521 mg/mL, water extract 0 . 8579 mg/mL, Acarbose 0 . 2593 mg/mL). The extracts of cinnamon leaves contain flavonoids, and phenolic acids and possessed scavenging effect on DPPH . Moreover, these extracts exhibited good inhibitory potential against α amylase and α glucosidase enzymes . The ethanol extract showed higher activity than successive water extract of Cinnamomum zeylanicum . According to these results Cinnamomum zeylanicum ethanolic leaf extract shows high amount of anti-diabetic properties than the water extract .
Determination of variation components of harvested rice seeds grown in selected areas of four grama niladari divisions in North Central Province
(Research Symposium on Pure and Applied Sciences, 2018 Faculty of Science, University of Kelaniya, Sri Lanka, 2018) Weerakoon, T.; Jayawardane, B. M.
During the last two decades, alarmingly high incidences of kidney failure and some associated deaths have become very significant in certain parts of Madawachchiya district and Uva Province and North Central Province. Since rice is the staple food of Sri Lankans, identification of variation components in rice seeds will be beneficial. Hence, this study aimed to find variation components in rice seeds grown in areas severely affected and not affected by Chronic Kidney Disease of unknown etiology (CKDu) in the North Central Province. After having a discussion with the farmers, rice seed samples of Bg 355 was collected from four grama niladari divisions, Karambankulama, Unagaswewa, Angunachchiya and Kirgollewa. Three samples from each division were randomly collected from different paddy fields which were treated with Compo and M-60 weedicides and the collected samples were stored for 6 months. Certain components of rice seeds such as starch, total flavonoids, free amino acids, crude fiber and crude protein content of rice seeds were tested. Free amino acids was analyzed by Moore and Stein method, flavonoids by Aluminum Chloride colorimetric method, crude fiber was analyzed by filtration method, starch by iodine test and crude protein amount was analyzed by Kjeldahal method. Triplicates (n=3) were done for each assay for each division. Since added fertilizer is common for all the samples, components which vary depend only on the geographical location within the province. Statistical analysis was done using Mini tab 18 version. According to statistical analysis, free amino acids and crude fiber content in Bg 355 rice seeds were comparatively higher in Kirigollewa (0.6750 ± 0.0636) and Angunachchiya (0.6600 ± 0.0283) areas severely affected by CKDu compared to Unagaswewa (0.3950 ± 0.0212) and Karambankulama (0.3800 ± 0.0141) areas less affected by CKDu. Total flavonoids were lesser in rice seeds in Kirigollewa (0.055 ± 0.007) and Angunachchiya (0.075 ± 0.007) areas severely affected by CKDu when compared to Unagaswewa (0.155 ± 0.007) and Karambankulama (0.135 ± 0.007) areas less affected by CKDu. Contents of starch and crude protein amount, does not show any significant difference related to CKDu affected and nonaffected areas. Therefore, this analysis suggests that free amino acids, total flavonoids and crude fiber contents in rice seeds vary depending on the geographical location within the North Central Province. Further experiments in different areas and geographical locations are needed to confirm the variation of these components depending on the geographical location.