Browsing by Author "Jayatilaka, K. A. P. W."

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Effect of a herbal capsule of Coccinia grandis (L.) Voigt (Cucurbitaceae) on lipid profile in patients with newly diagnosed type 2 diabetes mellitus
(Institute of Chemistry Ceylon Adamantane House, Rajagiriya, Sri Lanka., 2020) Wasana, K. G. P.; Attanayake, A. P.; Weerarathna, T. P.; Jayatilaka, K. A. P. W.
Herbal antidiabetic agents have been popular among general population due to their historical usage along with the exceptional therapeutic efficacy. The present study was aimed to determine the effect of a newly developed herbal capsule of C. grandis, which consists of freeze dried powder of the hot water extract of C. grandis leaves, on lipid profile in patients with newly diagnosed type 2 diabetes mellitus (T2DM). Three months long, placebo controlled, double blind, randomized clinical trial was conducted involving 147 individuals with newly diagnosed T2DM. Patients with known renal, liver, cardiac, respiratory, thyroid, psychiatric and any other chronic or acute diseases, and pregnant women were excluded. Individuals are using antilipidaemic drugs were also excluded. Selected individuals were randomly allocated into two groups of test and control. The test group received newly developed herbal capsule of C. grandis (500 mg) and the control group received placebo capsule (500 mg) once daily for three months. Serum lipid profile consisting of total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), triglyceride (TG) and very low density lipoprotein cholesterol (VLDL-C) was estimated at the base line (week=0) and at the end of intervention (week=12). Independent sample t-test was used to compare the two groups. A percentage reductions of TC, LDL-C, TG and VLDL-C were 2.12%, 3.51%, 27.36% and 27.36% respectively and increment of HDL-C (1.92%) was observed in herbal capsule treated group. The results of the t- test revealed that TG (p=0.003) and VLDL-C (p=0.003) are significantly decreased in the herbal capsule treated group. There were no statistically significant changes (p>0.05) in TC, HDL-C and LDL-C between two groups. In conclusion, 500 mg of herbal capsule of C. grandis is beneficial in reducing elevated TG and VLDL-C in lipid profile of patients with newly diagnosed T2DM.
In vitro antidiabetic activity of fractionated extracts of Coccinia grandis (L.) Voigt
(Faculty of Science, University of Kelaniya, Sri Lanka, 2021) Wasana, K. G. P.; Attanayake, A. P.; Jayasinghe, J. M. S.; Weeraratna, T. P.; Jayatilaka, K. A. P. W.
The Paspanguwa herbal formulation is commonly consumed as a traditional medicine in Sri Lanka. Paspanguwa consists of five ingredients, namely the rhizome of Zingiber officinale (Inguru), leaves and stem of Hedyotis corymbosa (Pathpadagam), dried berries of Solanum xanthocarpum (Katuwalbatu), dried stem of Coscinium fenestratum (Venivalgata), and dried seeds of Coriandrum sativum (Koththamalli). The importance and objective of this study was to prove the antioxidant and anti-inflammatory properties of traditionally used decotion, Paspanguwa claimed to have. In the present study, water extracts of the individual ingredient and the Paspanguwa decoction were screened for their total soluble phenolic content (TPC), total soluble flavonoid content (TFC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and their ability to inhibit protein denaturation (anti-inflammatory activity). The highest and the lowest TPC was seen in Coriander and ginger as 12.76 ± 1.00 and 7.89 ± 0.86 mg Gallic acid equivalent/g dry weight, respectively. The highest and the lowest TFC was seen in Katuwalbatu and Pathpadagam as 778.19 ± 1.40 and 282.14 ± 1.49 µg Catechin equivalent/g of dry weight, respectively. The lowest and the highest IC50 values for the DPPH assay was seen in Paspanguwa decoction and Katuwalbatu as 253.4 ± 8.2 and 609.7 ± 5.6 µg/mL, respectively, while the standard ascorbic acid showed 111.0 ± 6.1 µg/mL. The highest and lowest reducing power percentages were seen in Paspanguwa decoction and coriander as 94.74 ± 1.31 and 22.95 ± 0.96 while the standard ascorbic acid showed 109.89 ± 0.96. The ability to inhibit protein denaturation varied in the order of: Acetylsalicylic acid (standard) > Paspanguwa decoction > ginger > coriander > Venivalgata > Katuwalbatu > Pathpadagam at all the three concentrations (625, 1250, and 2500 µg/mL). These results suggest that Paspanguwa water extract is a good source of antioxidants with TFC and TPC with a higher ability to inhibit protein denaturation. Our findings corroborate with the previous in vitro studies of the antioxidant activity of Paspanguwa. However, our study is the first to reveal the anti-inflammatory action, total flavonoid content, and reducing power of the Paspanguwa herbal formula. Further, this study validated the use of Paspanguwa as a good source of antioxidants together with anti-inflammatory activity in traditional Ayurvedic medicine.