Browsing by Author "Dilhani, S."

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    Effect of Croton aromaticus leaf extracts in controlling Crown Rot disease of Embul banana.
    (International Research Symposium on Pure and Applied Sciences, 2017 Faculty of Science, University of Kelaniya, Sri Lanka., 2017) Dilhani, S.; Wimalasiri, S.; Abeywickrama, K.; Kannangara, S.
    Embul banana (Musa acuminata-AAB) is one of the major dessert fruits in Sri Lanka, widely consumed by all economic groups due to its small size and characteristic flavour. Although Embul banana has high potential for export market storage and export of this commodity over long distances is difficult in consequence of postharvest fungal diseases. The most common and serious postharvest disease that affect Embul banana is Crown Rot (CR). Use of synthetic fungicides is the widely used method in controlling postharvest diseases of fruits worldwide. The interest of finding natural bioactive components has increased due to the harmful effects of synthetic fungicides on environment and health. In the present study, antifungal activity of aqueous, hot water and ethanolic leaf extracts of Croton aromaticus in controlling CR disease of Embul banana was investigated in vivo. Embul banana hands (85-days mature) were treated with C. aromaticus aqueous, hot water 100% (v/v) and ethanolic leaf extracts (400 mg/ml) alone or in combination with alum (1%) or distilled water (control) were stored in modified atmosphere packaging at 12-14 0C for 14 days. Each treatment comprised of 4 replicates. In-package gases were analysed on initial day and thereafter up to 14 days. Physicochemical properties (pH, firmness, TSS, TA), sensory properties (peel colour, flesh colour, aroma, flavour, taste, overall acceptability), and Crown Rot disease severity were determined in ripening induced fruits after 7 and 14 days of storage period. Statistical analysis was done using the MINITAB 16 statistical package. Oxygen levels measured were observed to be amaintained at 2.2-4.4% while CO2 levels were maintained at 5.5-8.4% in all packages at the end of 14 day storage period. C. aromaticus ethanolic leaf extract (400 mg/mL) was the most effective extract in controlling crown rot disease of Embul banana compared to aqueous and hot water leaf extracts. Physicochemical properties of Embul banana treated with C. aromaticus leaf extract alone and in combination with alum were not significantly different compared to control except for TSS and TA. Most of the sensory properties were preferred by sensory panelists with score values of above 6 indicating the good quality of samples. C. aromaticus ethanolic leaf extract + alum in combination with modified atmosphere packaging and cold storage could be used as a potential safe way of controlling Crown Rot disease of Embul banana.
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    Evaluation of antifungal effect of Croton aromaticus on storage life extension of banana
    (2016) Dilhani, S.; Wimalasiri, S.; Abeywickrama, K.; Kannangara, S.
    Effect of Croton aromaticus aqueous, hot water and ethanolic leaf extracts alone and in combination with alum against banana crown rot causing fungal pathogens were investigated using 96 well plate bioassay and liquid bioassay in vitro. According to the results of 96 well plate assay, C. aromaticus aqueous extract successfully inhibited conidial germination of C. musae up to 96.9%. Percentage germination inhibition of F. proliferatum is less than that of C. musae. Hot water extract expressed higher conidial germination inhibition of F. proliferatum. Conidial germination of C. musae was 100% inhibited at the concentration of 600 (%w/v) of ethanoic extract and at the same concentration F. proliferatum was inhibited by 90.4 %. Alum was fungicidal against C. musae and L. theobromae. C. aromaticus leaf extract in combination with alum controlled F. proliferatum more effectively compared to leaf extract alone. During liquid bioassay, ethanolic leaf extract totally inhibited the mycelial growth of test pathogens at the concentration of 800 (%w/v). Among three tested extracts, ethanolic extract was most effective in inhibiting both spore germination and mycelial growth of crown rot causing fungal pathogens.