Browsing by Author "De Silva, A. P."

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Colonic diverticular disease revisited
(Taylor & Francis, 2024-12) De Silva, A. P.; Nuwanshika, N.; Dassanayake, U.; Niriella , M. A.; Ranasinghe, P.; De Silva, H. J.
INTRODUCTION Diverticular disease, including diverticulosis and diverticulitis, presents a significant health concern globally, with increasing prevalence in Western societies and emerging trends in Asia. The incidence of diverticulitis, is on the rise, leading to significant morbidity and healthcare costs. AREAS COVERED A literature search was conducted using the PubMed database, and studies published between 1995 and 2024 were selected based on their relevance to the overall understanding of disease. This review investigates the clinical spectrum, classification, and management strategies of diverticular disease, focusing particularly on evolving trends in diagnosis and treatment. Discussions regarding the prevalence of diverticulosis, the identification of risk factors associated with disease progression, recent advancements in research, and the utilization of biomarkers in disease monitoring and treatment decision-making are considered in detail. EXPERT OPINION The discourse on diverticular disease underscores the pressing need for tailored management strategies and innovative treatments. Understanding the intricacies of the disease’s pathophysiology is paramount for effective intervention. Recent advances in diagnostic imaging and biomarker identification are promising, yet more research is imperative to further refine patient care. Advances in these areas hold the potential for significantly improving outcomes in disease management.
Development of an lc-ms/ms method for the detection of prednisolone doping using human fingernails
(Postgraduate Institute of Science (PGIS) , University of Peradeniya, 2024-11) Kanishka, W. A. S.; Fernando, P. N. J.; Rashani, S. A. N.; Jayakody, R. S.; Cooray, A.; De Silva, A. P.
Sports doping detection mainly relies on urine and blood samples. Nails are less commonly used, even though they can provide long-term drug use, due to challenges in preparation and pulverization. This study developed a Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) method to detect prednisolone in human fingernails. Nails from volunteers not administered with prednisolone were ethically collected, cleaned, dried, and pulverized for analysis. The extraction of prednisolone was optimized for solvent type, extraction frequency, and shaking rate. The optimized sample preparation was followed by cleaning of Nail clippings by vortexing at 2200 rpm using ultrapure water at 50 °C for 75 secs, drying at 50 °C for 80 mins, cutting into small pieces, and subjected to methanol extraction by shaking for 18 hrs at 300 rpm. LC-MS/MS analysis used a biphenyl column (100 mm × 2.1 mm, 2.7 μm) with a binary gradient of acidified (0.1% formic acid) acetonitrile and an aqueous phase to identify prednisolone. The run time for the LC-MS/MS method was 7.50 minutes. The retention time of prednisolone was 2.18 minutes. The method was validated according to International Council for Harmonization (ICH) guidelines, showing strong linearity (R² > 0.99) and high specificity with no carryover. Within-run accuracy and precision were ± 3.24% and 3.76% coefficient of variation (CV), respectively, while between-run accuracy and precision were ± 4.18% and 3.5% CV, respectively. LOD and LOQ were 0.0003 mg/L and 0.00154 mg/L, respectively. Using a cost-effective pulverization setup, the validated The LC-MS/MS method effectively detected prednisolone in fingernails. Further studies with prednisolone-doped nails are needed to advance nail-based doping detection in sports and forensics.
Screening for prednisolone in anti-asthmatic herbal remedies using lc-ms/ms in sri lanka.
(Postgraduate Institute of Science (PGIS), University of Peradeniya, 2024-11) Fernando, S. S. D.; Fernando, P. N. J.; Rashani, S. A. N.; Cooray, A.; De Silva, A. P.
Prednisolone, a corticosteroid used to treat asthma, poses significant concerns when adulterated in herbal remedies, particularly for athletes subjected to anti-doping regulations. Despite their widespread use, comprehensive studies to determine the extent of prednisolone adulteration in herbal products are lacking in Sri Lanka. This study aimed to fill this research gap by screening commonly used herbal remedies for asthma in Sri Lanka to identify the presence of Prednisolone. A tailored LC-MS/MS method was developed and validated for this purpose. An optimized ultrasound-assisted extraction procedure was established using a reference standard, achieving a recovery rate of 100.45% by adjusting the extraction solvent and sonication time. The LC-MS/MS method utilised a biphenyl column (100 mm × 2.1 mm, 2.7 μm) with a mobile phase of acetonitrile and water acidified with 0.1% formic acid. Chromatographic conditions were optimized for a column; oven temperature of 50 °C, an initial acetonitrile concentration of 5%, a dwell time of 0.200 secs, and a run time of 10 min. Validation of the method followed EuraChem guidelines and was done using a Prednisolone reference standard, demonstrating high precision, accuracy, sensitivity, and specificity. Extraction recoveries showed relative standard deviation (%RSD) ≤ 10% and calibration linearities had R2 ≥ 0.988. The detection limits were 0.03 μg/g for powdered and 0.003 μg/mL for liquid herbal matrices. The study screened 27 different over-the-counter brands and prescription anti-asthmatic herbal products collected randomly from suburban areas of Colombo. The validated LC-MS/MS method identified that 25 products were below the detection limit, while two prescribed products were flagged as doubtful. In conclusion, the developed LC-MS/MS method successfully detected prednisolone in complex herbal matrices with high specificity and precision, providing valuable insights into the prevalence and extent of prednisolone adulteration in herbal remedies.