Browsing by Author "De Silva, A. G. K. S."

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    Antimicrobial potential of different Aporosa spp. (S. Kebella) in Sri Lanka
    (Faculty of Science, University of Kelaniya Sri Lanka, 2024) De Silva, A. G. K. S.; Thathsarani, Y. K. D.D.; Senanayake, S. P.
    The genus Aporosa belongs to the family Phyllanthaecae and locally referred to as ‘S: Kebella’ is reported to be rich with medicinal properties. Despite sharing the same vernacular name, these plant species exhibit significant variation in morphological traits leading drug adulteration. This suggests establishment of accurate identification methods of these species while assessing their potential medicinal properties. The present study was focused to evaluate the antimicrobial activity of ethanolic extract of the leaves of five Aporosa species found in Sri Lanka and thereby revealing the best plant/s with the inhibition properties of pathogenic microorganisms. Five Crude extracts of authenticated plant specimens were prepared using Dimethyl sulfoxide (DMSO). Antibacterial activity was evaluated against Escherichia coli and Staphylococcus aureus, while antifungal activity was tested against Candida albicans. The well diffusion method was employed across four extract concentrations (0.5, 1, 2, 4 mg/mL) and the results revealed concentration-dependent effects. Microorganisms were quantitatively assessed by the presence or absence of inhibition zones. Clear inhibition zones indicated the compounds showed antimicrobial activity against the tested microorganisms. According to the results all the tested extracts have produced zones of inhibitions against E. coli indicating their antibacterial activity against E. coli. High potential of antibacterial activity against E. coli was shown by Aporosa cardiosperma morphotype 3 at the concentrations of 4.00 mg/ mL and 2.00 mg/mL, with their respective diameter zones of inhibition ranging from 14.70–16.00 mm. Next highest inhibition zone of 13.70 was shown by A. cardiosperma at the concentrations of 1.00 mg/mL, 2.00 mg/mL, 4.00 mg/mL. However, S. aureus has shown response to none of the tested leaf extracts in all concentrations. All the extracts have produced zones of inhibitions against C. albicans indicating their antifungal activity against C. albicans. High potential of antifungal activity against C. albicans was shown by A. cardiosperma morphotype 2 at the concentrations of 2.00 mg/ mL and 4.00 mg/mL, with their respective diameter zones of inhibition ranging from 15.70–16.70 mm. The next highest inhibition zones ranging from 14.0–15.30 mm were shown by both A. cardiosperma morphotype 1 and A. cardiosperma at the concentrations of 1.00 mg/mL, 2.00 mg/mL, 4.00 mg/mL. Comparative analysis with standard antibiotics revealed distinct efficacy profiles. Notably, Aporosa extracts showed considerable inhibitory effects although smaller than antibiotics. The findings of the research concluded the potential of utilizing the tested Aporosa spp. for their antimicrobial properties, underlying their capabilities in combating pathogenic microbes. These findings underscore the therapeutic promise of leaf extracts of Aporosa spp. in antimicrobial applications, warranting further exploration for pharmaceutical development.