de Silva, B.G.D.N.K.Hapugoda, G.P.G.M.D.Karunanayake, E.H.2014-10-292014-10-292010Vidyodaya Journal of Science. 2010; 15(1): pp.63-72http://repository.kln.ac.lk/handle/123456789/1995Three cloned highly repetitive DNA sequences Rp36, Rp217 and Rp234 isolated from An. culicifacies Giles, sensu lato were developed as non radioactive DNA probes by using a biotinylated labeling and colorimetric detection system. These non radioactive DNA probes distinguish sibling species A from species B and C of the An. culicifacies complex in a dot blot hybridization assay using single mosquito DNA extracts diluted 50 fold. The biotinylated Rp217 probe was further assayed in a more simple procedure which involves the hybridization of blots prepared from squashed mosquito heads. This technique avoids the separate extraction of mosquito DNA and facilitates a number of samples to be processed rapidly while also allowing several field analyses to be carried out on one mosquito specimen.ArticleMolecular Medicine Unit