Pathiratne, A.Hemachandra, C.K.Pathiratne, K.A.S.2014-11-192014-11-192010Bulletin of Environmental Contamination and Toxicology, May 2010, Volume 84, Issue 5, pp 554-558http://repository.kln.ac.lk/handle/123456789/4361Bile fluorescence patterns in Nile tilapia, a potential fish for biomonitoring tropical water pollution were assessed following exposure to selected polycyclic aromatic hydrocarbons (PAHs): naphthalene, phenanthrene, pyrene and chrysene. Non-normalized fixed wavelength fluorescence signals in the fish exposed to these PAHs reflected dose and/or time response relationships of their metabolism. Normalizing signals to biliverdin introduced deviations to these response patterns. The optimal wavelength pairs (excitation/emission) for synchronous fluorescence scanning measurements of bile metabolites of naphthalene, phenanthrene, pyrene and chrysene were identified as 284/326, 252/357, 340/382 and 273/382 respectively. This study supports the use of bile fluorescence in Nile tilapia by fixed wavelength fluorescence and synchronous fluorescence spectrometry with non-normalized data as a simple method for screening bioavailability of these PAHs.Tropical FishSri Lanka;Nile tilapia (Oreochromisniloticus);Phenanthrene; Pyrene (Chemical); Synchronous generatorsarticleFisheries Biology and Aquaculture