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http://repository.kln.ac.lk/handle/123456789/16184
Title: | In situ immunopathological changes in cutaneous leishmaniasis due to Leishmania donovani |
Authors: | Manamperi, N.H. Oghumu, S. Pathirana, N. de Silva, V.C. Abeyewickreme, W. Satoskar, A.R. Karunaweera, N.D. |
Keywords: | Leishmaniasis Leishmaniasis, Cutaneous Leishmaniasis, Cutaneous - immunology Leishmania donovani |
Issue Date: | 2017 |
Publisher: | Oxford, Wiley |
Citation: | Parasite Immunology. 2017; 39(3): e12413 |
Abstract: | INTRODUCTION: Cutaneous leishmaniasis in Sri Lanka is a newly established parasitic disease caused by the usually visceralizing Leishmania donovani. Skin lesions manifest as non-itchy, non-tender papules, nodules or ulcers. In situ cytokine expression provides clues for immunopathogenesis of this localized form of disease. METHODS: Skin biopsies from 58 patients were analyzed for histological appearance and in situ cytokine expression of T- helper 1 (Th1) and T- helper 2 (Th2) cytokines, namely interferon (IFN)-γ, interleukin (IL)-12A, tumor necrosis factor (TNF)-α, IL-4 and IL-10 by real-time RT- PCR. RESULTS: Significant up regulation of the Th1 cytokine IFN-γ and down regulation of the Th2 cytokine IL-4 was seen in patients compared to healthy controls. Significantly elevated tissue expression of IFN-γ and TNF-α was seen in lesions that presented later than 6 months from the time of onset, while IL-4 expression was more prominent in lesions that responded poorly to antimony therapy. CONCLUSION: A prominent Th1 response appears to support resolving of lesions, whereas a Th2 biased milieu tends to favor poor responsiveness to antimony and delayed lesion healing in L. donovani infections in Sri Lanka. This article is protected by copyright. All rights reserved. |
Description: | Indexed in MEDLINE |
URI: | http://repository.kln.ac.lk/handle/123456789/16184 |
ISSN: | 0141-9838 (Print) 1365-3024 (Electronic) 0141-9838 (Linking) |
Appears in Collections: | Journal/Magazine Articles |
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InSituIm.2017.pdf | 676.39 kB | Adobe PDF | View/Open |
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