A Simplified non radioactive DNA probe technique for the field detection of sibling species A of the Anopheles culicifacies (Diptera: Culicidae) complex
| dc.contributor.author | de Silva, B.G.D.N.K. | en_US |
| dc.contributor.author | Hapugoda, G.P.G.M.D. | en_US |
| dc.contributor.author | Karunanayake, E.H. | en_US |
| dc.creator.corporateauthor | University of Sri Jayewardenepura | en_US |
| dc.date.accessioned | 2014-10-29T09:31:59Z | |
| dc.date.available | 2014-10-29T09:31:59Z | |
| dc.date.issued | 2010 | en_US |
| dc.description.abstract | Three cloned highly repetitive DNA sequences Rp36, Rp217 and Rp234 isolated from An. culicifacies Giles, sensu lato were developed as non radioactive DNA probes by using a biotinylated labeling and colorimetric detection system. These non radioactive DNA probes distinguish sibling species A from species B and C of the An. culicifacies complex in a dot blot hybridization assay using single mosquito DNA extracts diluted 50 fold. The biotinylated Rp217 probe was further assayed in a more simple procedure which involves the hybridization of blots prepared from squashed mosquito heads. This technique avoids the separate extraction of mosquito DNA and facilitates a number of samples to be processed rapidly while also allowing several field analyses to be carried out on one mosquito specimen. | |
| dc.identifier.citation | Vidyodaya Journal of Science. 2010; 15(1): pp.63-72 | en_US |
| dc.identifier.department | Molecular Medicine Unit | en_US |
| dc.identifier.uri | http://repository.kln.ac.lk/handle/123456789/1995 | |
| dc.publisher | University of Sri Jayewardenepura | en_US |
| dc.title | A Simplified non radioactive DNA probe technique for the field detection of sibling species A of the Anopheles culicifacies (Diptera: Culicidae) complex | en_US |
| dc.type | Article | en_US |
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