Bioprospecting of an Endolichenic Fungus Phanerochaete sordida Isolated from Mangrove-Associated Lichen Bactrospora myriadea
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Date
2022
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Journal of Chemistry
Abstract
Bioassay-guided fractionation of the ethyl acetate extract of Phanerochaete sordida, an endolichenic fungus (ELF) isolated from
the host lichen Bactrospora myriadea, collected from Negombo lagoon, Sri Lanka, led to the isolation of a bioactive compound.
Following the identification of the fungus using morphological and DNA barcoding techniques, the pure compound was
isolated using column chromatography, preparative TLC, and semipreparative HPLC. The structure elucidation was carried out
using IR, HR-ESI-MS and 1H, 13C & 2D NMR spectroscopic methods. The in vitro bioassays conducted revealed that compound 1
has a high antioxidant activity with ABTS•+ (IC5058:91 ± 1:35 μM), moderate anti-inflammatory activity (IC50254:79 ± 1:41 μM),
comparable antibacterial activity against the oral-bacterial strain Streptococcus mutans (MIC 898.79 μM and MLC 1797.58μM),
moderate tyrosinase inhibition (IC501713:69 ± 8:65 μM), and moderate cytotoxicity against oral cancer (IC5013:65 ± 0:02 μM), in
comparison with respective positive controls. The in silico experiments conducted for tyrosinase inhibition and cytotoxicity using
Schrödinger revealed results in line with the in vitro results, thus confirming the bioactivities. The molecule also satisfies the key
features of drug likeliness according to pharmacokinetic studies.
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Weerasinghe, R. H., Shevkar, C. D., Maduranga, K., Pandey, K. H., Attanayake, R. N., Kate, A. S., Weerakoon, G., Behera, S. K., Kalia, K. S., & Paranagama, P. A. (2022). Bioprospecting of an Endolichenic Fungus Phanerochaete sordida Isolated from Mangrove-Associated Lichen Bactrospora myriadea. Journal of Chemistry, 2022, 1–11. https://doi.org/10.1155/2022/3193689