Citation:Iqbal, Asif, Sohag, Mehadi Hasan, Ahmed, Jahed, Rana, Mohosin, Rakibuzzaman, S.M., Islam, Kamrul and Azad, Abul Kalam, 2015. Production of industrially important enzymes and microbial lipids from biomass of Municipal Solid Wastes (MSW) in Bangladesh. Proceedings of the International Workshop on Molecular Biology Techniques Related to Infectious/Genetic Diseases & Human Identification, Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya.
Date:2015
Abstract:
Introduction: Municipal Solid Wastes (MSW) in developing countries is usually
dumped in open field resulting environmental pollution and public health hazards.
Methane, the second prevalent greenhouse gas having impact on climate change
over 20 times greater than carbon dioxide is emitted from the rotten MSW. Organic
part which is almost 70-80% of MSW can be converted to bioresources to mitigate
the climate change and environmental pollution.
Objective: To produce industrially important enzymes and microbial lipids from
biomass of MSW.
Methodology: We have isolated and identified proteolytic and cellulolytic bateria
and fungi from MSW, cowdung and sea sands. We have successfully used MSW
as raw materials in the bioreactor for production of industrially important protease
and cellulase enzymes from bacterial and fungal isolates and microbial lipids from
oliagenous yeast Lipomyces starkeyi. The enzymes were purified and
characterized.
Results: Amount of accumulated lipids of L. starkeyi grown in MSW hydrolysate
media under optimal conditions was ~45% (v/w) of dry biomass of the yeast cells.
Gas chromatographic analysis revealed that this lipid was composed of saturated
and unsaturated fatty acids. The fatty acid composition of this microbial lipid is
similar to that of vegetable oils.
Discussion: Our study indicates that organic MSW might be a valuable alternative
feedstock for production of microbial lipids and industrially important enzymes.