dc.contributor.author |
Kim, H. K. |
|
dc.contributor.author |
Premaratna, R. |
|
dc.contributor.author |
Missiakas, D. M. |
|
dc.contributor.author |
Premaratna, R. |
|
dc.date.accessioned |
2019-08-20T09:17:40Z |
|
dc.date.available |
2019-08-20T09:17:40Z |
|
dc.date.issued |
2019 |
|
dc.identifier.citation |
Proceedings of the National Academy of Sciences of the United States of America.2019;116(39):19659-19664. |
en_US |
dc.identifier.issn |
0027-8424 (Print) |
|
dc.identifier.issn |
1091-6490 (Electronic) |
|
dc.identifier.uri |
http://repository.kln.ac.lk/handle/123456789/20349 |
|
dc.description |
indexed in MEDLINE. |
en_US |
dc.description.abstract |
Rickettsial diseases have long been diagnosed with serum antibodies cross-reactive against Proteus vulgaris (Weil-Felix reaction). Although Weil-Felix antibodies are associated with the development of immunity, their rickettsial target and contribution to disease pathogenesis are not established. Here, we developed a transposon for insertional mutagenesis of Rickettsia conorii, isolating variants defective for replication in cultured cells and in spotted fever pathogenesis. Mutations in the polysaccharide synthesis operon (pso) abolish lipopolysaccharide O-antigen synthesis and Weil-Felix serology and alter outer-membrane protein assembly. Unlike wild-type R. conorii, pso mutants cannot elicit bactericidal antibodies that bind O antigen. The pso operon is conserved among rickettsial pathogens, suggesting that bactericidal antibodies targeting O antigen may generate universal immunity that could be exploited to develop vaccines against rickettsial diseases. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Washington, DC : National Academy of Sciences |
en_US |
dc.subject |
O antigen |
en_US |
dc.title |
Rickettsia conorii O antigen is the target of bactericidal Weil-Felix antibodies |
en_US |
dc.type |
Article |
en_US |