| dc.contributor.author |
de Silva, B.G.D.N.K. |
en_US |
| dc.contributor.author |
Hapugoda, G.P.G.M.D. |
en_US |
| dc.contributor.author |
Karunanayake, E.H. |
en_US |
| dc.date.accessioned |
2014-10-29T09:31:59Z |
|
| dc.date.available |
2014-10-29T09:31:59Z |
|
| dc.date.issued |
2010 |
en_US |
| dc.identifier.citation |
Vidyodaya Journal of Science. 2010; 15(1): pp.63-72 |
en_US |
| dc.identifier.uri |
http://repository.kln.ac.lk/handle/123456789/1995 |
|
| dc.description.abstract |
Three cloned highly repetitive DNA sequences Rp36, Rp217 and Rp234 isolated from An. culicifacies Giles, sensu lato were developed as non radioactive DNA probes by using a biotinylated labeling and colorimetric detection system. These non radioactive DNA probes distinguish sibling species A from species B and C of the An. culicifacies complex in a dot blot hybridization assay using single mosquito DNA extracts diluted 50 fold. The biotinylated Rp217 probe was further assayed in a more simple procedure which involves the hybridization of blots prepared from squashed mosquito heads. This technique avoids the separate extraction of mosquito DNA and facilitates a number of samples to be processed rapidly while also allowing several field analyses to be carried out on one mosquito specimen. |
|
| dc.publisher |
University of Sri Jayewardenepura |
en_US |
| dc.title |
A Simplified non radioactive DNA probe technique for the field detection of sibling species A of the Anopheles culicifacies (Diptera: Culicidae) complex |
en_US |
| dc.type |
Article |
en_US |
| dc.identifier.department |
Molecular Medicine Unit |
en_US |
| dc.creator.corporateauthor |
University of Sri Jayewardenepura |
en_US |