dc.contributor.author |
Samarawickrema, N.A. |
en_US |
dc.contributor.author |
Brown, D.M. |
en_US |
dc.contributor.author |
Upcroft, J.A. |
en_US |
dc.contributor.author |
Thammapalerd, N. |
en_US |
dc.contributor.author |
Uproft, P. |
en_US |
dc.date.accessioned |
2014-10-29T09:14:59Z |
|
dc.date.available |
2014-10-29T09:14:59Z |
|
dc.date.issued |
1997 |
en_US |
dc.identifier.citation |
The Journal of Antimicrobial Chemotherapy. 1997; 40: pp.833-840 |
en_US |
dc.identifier.issn |
0305-7453 (Print) |
en_US |
dc.identifier.issn |
1460-2091 (Electronic) |
en_US |
dc.identifier.uri |
http://repository.kln.ac.lk/handle/123456789/1304 |
|
dc.description |
Indexed in MEDLINE |
|
dc.description.abstract |
Metronidazole resistance has been induced in an axenic strain of Entamoeba histolytica (HTH-56:MUTM) following continuous exposure to steadily increasing drug concentrations. The drug-resistant line is routinely maintained in normally lethal levels of metronidazole (10 microM).Resistance to this concentration of drug was developed over 177 days. Decreased pyruvate:ferredoxin oxidoreductase (PFOR) activity in anaerobic organisms is one mechanism of metronidazole resistance but in entamoeba, PFOR activity was not decreased in metronidazole-resistant parasites as determined by immunofluorescent assays and immunoblotting studies. 2-Oxoacid oxidoreductase activity, which appeared to be due to a single enzyme, PFOR, was evident with pyruvate as well as the alternative substrates, alpha-ketobutyrate, alpha-ketoglutarate and oxaloacetate. A marked increase in superoxide dismutase (SOD) activity was detected in metronidazole-resistant E.histolytica. Increased SOD activity has not previously been documented as a mechanism of drug resistance although SOD has been associated with a range of stress situations in other organisms. |
en_US |
dc.publisher |
Oxford University Press |
en_US |
dc.subject |
Drug Resistance |
en_US |
dc.subject |
Entamoeba histolytica |
en_US |
dc.subject |
Entamoeba histolytica-drug effects |
en_US |
dc.subject |
Entamoeba histolytica-enzymology |
en_US |
dc.subject |
Ketone Oxidoreductases-metabolism |
en_US |
dc.subject |
Metronidazole |
en_US |
dc.subject |
Metronidazole-pharmacology |
en_US |
dc.subject |
Superoxide Dismutase-metabolism |
en_US |
dc.subject |
Comparative Study |
en_US |
dc.title |
Superoxide dismutase and pyruvate ferrodoxin oxidoreductase involvement in mechanisms of metronidazole resistance in Entamoeba histolytica |
en_US |
dc.type |
Article |
en_US |
dc.identifier.department |
Biochemistry |
en_US |
dc.creator.corporateauthor |
British Society for Antimicrobial Chemotherapy |
en_US |