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Effects of high external concentrations of K+ on 86Rb+ efflux in human platelets: evidence for Na+/K+/2Cl- co-transport.

Show simple item record de Silva, H.A. en_US Carver, J.G. en_US Aronson, J.K. en_US 2014-10-29T09:14:44Z 2014-10-29T09:14:44Z 1996 en_US
dc.identifier.citation Clinical Science. 1996; 91(6): pp.725-731 en_US
dc.identifier.issn 0143-5221 (Print) en_US
dc.identifier.issn 1470-8736 (Electronic) en_US
dc.description Indexed in MEDLINE
dc.description.abstract Na+/K+/2Cl- co-transport mediates a bidirectional symport of Na+, K+ and Cl-. The important properties of the co-transport system are its requirement for Na+, K+ and Cl- and its inhibition by loop diuretics such as bumetanide. This co-transporter has been described in a number of animal and human tissues. However, its presence in human platelets, although inferred, has not been demonstrated directly. 2. We have studied the efflux of 86Rb+ (a marker for K+) from Rb(+)-loaded platelets, and have defined their response to stimulation by high concentrations of external K+. 3. KCl (30-120 mmol/l) stimulated a concentration-dependent increase in 86Rb+ efflux from the platelets. This efflux was completely inhibited by bumetanide (10 mumol/l) but was insensitive to ouabain and R(+)-[(dihydroindenyl)oxy]alkanoic acid. It also required Cl- in the external medium, but did not depend on the presence of extracellular Na+. 4. These observations suggest that 86Rb+ efflux from platelets stimulated by external K+ occurs via Na+/K+/2Cl- co-transport acting in a K+/K+ (K+/Rb+) exchange mode. 5. Non-stimulated efflux of 86Rb+ from the platelets (i.e. in the presence of 5 mmol/l K+) had the characteristics of Na+/K+/2Cl- co-transport acting in normal mode.
dc.publisher Portland Press on behalf of the Medical Research Society and the Biochemical Society en_US
dc.subject Biological Transport-drug effects
dc.subject Blood Platelets-metabolism
dc.subject Bumetanide-pharmacology
dc.subject Carboxylic Acids-pharmacology
dc.subject Chlorine-blood
dc.subject Indenes-pharmacology
dc.subject Ouabain-pharmacology
dc.subject Potassium-blood
dc.subject Rubidium Radioisotopes-blood
dc.subject Sodium-blood
dc.title Effects of high external concentrations of K+ on 86Rb+ efflux in human platelets: evidence for Na+/K+/2Cl- co-transport. en_US
dc.type Article en_US
dc.identifier.department Pharmacology en_US
dc.creator.corporateauthor Biochemical Society (Great Britain) en_US
dc.creator.corporateauthor Medical Research Society (Great Britain) en_US
dc.creator.corporateauthor International Society of Hypertension en_US

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