dc.contributor.author |
Chandrasekharan, N.V. |
en_US |
dc.contributor.author |
Karunanayake, E.H. |
en_US |
dc.contributor.author |
Franzen, L. |
en_US |
dc.contributor.author |
Abeyewickreme, W. |
en_US |
dc.contributor.author |
Pettersson, U. |
en_US |
dc.date.accessioned |
2014-10-29T09:11:25Z |
|
dc.date.available |
2014-10-29T09:11:25Z |
|
dc.date.issued |
1994 |
en_US |
dc.identifier.citation |
Experimental Parasitology. 1994; 78(3): pp.279-286 |
en_US |
dc.identifier.issn |
0014-4894 (Print) |
en_US |
dc.identifier.issn |
1090-2449 (Electronic) |
en_US |
dc.identifier.uri |
http://repository.kln.ac.lk/handle/123456789/1215 |
|
dc.description |
Indexed in MEDLINE |
|
dc.description.abstract |
A highly repetitive DNA element from the genome of the filarial nematode Dirofilaria repens has been cloned and sequenced. The 176-base pair repeating units are arranged in direct tandem and are clustered in the parasite genome. All repeats appear to belong to a single family although some elements have diverged significantly. The repeats are present in about 15,000 copies and constitute approximately 3.0% of the parasite genome. The cloned repetitive sequence hybridized only to D. repens DNA and was sensitive enough to detect 250 to 500 pg of D. repens DNA, a single microfilariae in infected blood samples, and a single third stage larvae in mosquitoes. The high specificity and sensitivity of the cloned fragment makes it ideal as a diagnostic probe for detecting D. repens in both the host and the vector. |
en_US |
dc.publisher |
Academic Press |
en_US |
dc.subject |
Dirofilariasis |
|
dc.subject |
Dirofilaria-genetics |
|
dc.subject |
Dirofilaria-isolation and purification |
|
dc.subject |
Dirofilariasis-diagnosis |
|
dc.subject |
Dog Diseases-diagnosis |
|
dc.subject |
DNA-analysis |
|
dc.subject |
DNA-chemistry |
|
dc.subject |
DNA Probes-chemistry |
|
dc.subject |
Repetitive Sequences, Nucleic Acid |
|
dc.title |
Dirofilaria repens: Cloning and Characterization of a Repeated DNA Sequence for the Diagnosis of Dirofilariasis in Dogs, Canis familiaris |
en_US |
dc.type |
Article |
en_US |
dc.identifier.department |
Parasitology |
en_US |